• Istituto di Biologia e Patologia Molecolari

Yeast Saccharomyces Cerevisiae

Yeast relevance is related to the easy determination of the link between gene and protein function  (Botstein and Fink 1988).
Based on the high evolutionary conservation Yeast is an ideal model system where the study of genomic regulation and molecular mechanisms can be extended as working models to human cells. The correlation between a gene and phenotype and the possibility to recapitulate complex gene networks allow to study complex functions in humans.
Main features
• S.cerevisiae shows an aploid 
• Yeast Saccharomyces cerevisiae genome (12-068Kb) has been fully sequenced in 1996 and code for about 5885 genes. 
• Homology recombination allows easy disruption of selcted genes. 
• Gene deletions and/or mutatios can be studied in the aploid or diploid strain 
• High variety of experimental protocols are used to explain the correlation between a given gene and the phenotype 

OMIC technology
Big database (SGD) provide experimental data obtained by the international scientific community and offer an awesome platform with useful informations allowing to find genes and genomic functions conserved in Evolution. 
• genetic and molecular experimental data
• collections of mutant and deltion strains
• genetic and biophysical gene networks 
• gene expression profiles

Patrizia Filetici




~ 500 strains of budding yeast Saccharomyces cerevisiae  
genetic background,  
W303: ade2-1, trp1-1, leu2-3, 112 his3-11,15, ura3, can1-100, ssd1.
Collection of mutants, disrupted and tagged strains in epigenetic modulators such as acetylatrnsferases, ubiquitin proteases, individual subunits of multiproteic chromatin remodeling complex i.e. SAGAc. In the collection are present also double and triple disrupted strains for genetic analysis and epistatic assay. 
disrupted strains: KAT-Gcn5, DUB-Ubp8, Sgf73, Sus1, Ada2, Ada3, Spt7, Spt20
tagging: Gcn5-Myc, Ubp8-Myc, Gcn5-GFP, Cse4-myc, 
strains expressing His6-Ub for the biochemical purification of Ub-proteins.
TS mutants of kinetochore components, mitotic checkpoint and microtubule binding proteins associated to GCN5 or UBP8 disruptions.
Contact P.Filetici (patrizia.filetici@uniroma1.it)