Collections, engineered stocks and cell lines @IBPM
IBPM is a partner of Addgene (https://www.addgene.org), a non-profit plasmid repository that allows deposit and retrieval of plasmids and other vectors.
Human cell line collection
Pathway: RNA processing - E. Caffarelli (elisa.caffarelli@uniroma1.it)
Cell line: Human Neuroblastoma SH-SY5Y cells
Transgene: shRNA-TDP43
System: tet on (inducible loss of function)
Pathway: neuronal differentiation, apoptosis - E. Caffarelli (elisa.caffarelli@uniroma1.it)
Cell line: Human Neuroblastoma SKNBE cells
Transgene: Linc-NeD125 lncRNA
System: tet on (inducible gain of function)
Pathway: myc pathway - B. Illi. (barbara.illi@uniroma1.it)
Cell line: HEK293T cells, glioblastoma U87MG cells and glioblastoma stem cells
Transgene: FlagOmomyc expressing lentiviral vector
System: tet on (inducible gain of function)
Cell line: HEK293T and glioblastoma stem cells
Transgene: Myc shRNA expressing lentiviral vector .
System: tet on
Pathway: centrosomes - G.Guarguaglini (giulia.guarguaglini@uniroma1.it)
Cell line: human epithelial cells immortalized with hTERT (hTERT-RPE1)
Transgene: Aurora-A and/or TPX2
system: tet on (inducible overexpression of full length and deletion mutants)
Pathway: kinetochores - F. Degrassi (francesca.degrassi@uniroma1.it)
Cell line: HeLa human cervical cancer cells
Transgene: Hec1
system: tet on (inducible expression of N-ter GFP-tagged Hec1 or C-ter GFP-tagged Hec1)
Pathways: nuclear transport; mitosis - P. Lavia (patrizia.lavia@uniroma1.it)
Cell line: HeLa human cervical cancer cells
Transgene: Importin beta-1 (KPNB-1) or Chromosome Mantainance-1/Exportin-1 (CRM1/XPO1)
System: tet on (inducible expression of EGFP-tagged Importin beta-1 or EGFP-tagged CRM1)
Cell line: HeLa human cervical cancer cells, containing the following transgenes:
- Importin beta-1 wild-type (KPNB-1wt)
- Importin beta-1 nucleoporin-binding defective (KPNB I178A/Y255A)
- Chromosome Mantainance-1/Exportin-1 (CRM1/XPO1)
- Control cell line: HeLa EGFP
Expression system in all cell lines: tet on (inducible expression of EGFP-tagged tansgenes)
Cell line: RPE-1 human non-transformed retinal epithelial cells, containing the following transgenes:
- Importin beta-1 wild-type (KPNB-1wt)
- Chromosome Mantainance-1/Exportin-1 (CRM1/XPO1)
- Control cell line: RPE-1 EGFP
System: tet on (inducible expression of EGFP-tagged transgenes)
Cell line: Human fibrosarcoma HT1080 cells
Transgene: eGFP-LC3 or RPF- eGFP-LC3
System: CMV promoter
Budding yeast mutant strains - Saccharomyces cerevisiae
Contact Filetici P. patrizia.filetici@uniroma1.it
> 500 strains of budding yeast Saccharomyces cerevisiae
genetic background,
W303 ade2-1, trp1-1, leu2-3, 112 his3-11,15, ura3, can1-100, ssd1.
Collection of mutants, disrupted and tagged strains in epigenetic modulators. e.g.
- acetylatrnsferases,
- ubiquitin proteases,
- individual subunits of multiproteic chromatin remodeling complex (SAGAc).
The collection also features double and triple disrupted strains for genetic analysis and epistatic assays.
Disrupted strains: KAT-Gcn5, DUB-Ubp8, Sgf73, Sus1, Ada2, Ada3, Spt7, Spt20
tagging: Gcn5-Myc, Ubp8-Myc, Gcn5-GFP, Cse4-myc,
strains expressing His6-Ub for the biochemical purification of Ub-proteins.
TS mutants for kinetochore components, mitotic checkpoint and microtubule-binding proteins, associated to GCN5 or UBP8 disruptions.
Plants - Arabidopsis thaliana
Loss- and gain-of-function HD-Zip II transcription factor mutants
HD-Zip II constitutive and inducible overexpressing lines
HA-, GFP- and YFP-tagged HD-Zip II proteins
Combinations of mutants belonging to different HD-Zip protein families (HD-Zip II, HD-Zip III)
Root, shoot and flower markers introgressed in loss-of-function HD-Zip II mutants
HD-Zip II markers introgressed in root, shoot and flower regulatory mutants
Combinations of HD-Zip II mutants and root, shoot and flower regulatory mutants
Auxin markers introgressed in loss-of-function HD-Zip II mutants
Combinations of HD-Zip II mutants and auxin transport/signalling mutants
contact maura.cardarelli@uniroma1.it
Light signalling mutants
Auxin perception mutants
Auxin transport mutants
Auxin conjugate synthetase mutants
Loss-of-function ARF (auxin-response factor) mutants
ARF inducible overexpressing lines
PIN and ABCB (auxin transport) inducible overexpressing lines
DR5:GUS introgressed into the wild type and auxin perception mutant backgrounds
DR5:GFP introgressed into the wild type and auxin trasnport mutant backgrounds
Mouse model collection
Transgenic mice
Mouse model: Transgenic mouse named Tg-Jazz (CNR patented)
Genetic background: generated on the Hybrid genetic background BDF1 (C57Black6 x DBA)
Transgene: Zinc Finger Artificial Transcription Factor ZF-ATF “Jazz” (GenBank accession number AJ243577.1.)
Expression: muscle-specific
Contacts: Claudio Passananti (IBPM): claudio.passananti@uniroma1.it
Elisabetta Mattei (IBCN): elisabetta.mattei@cnr.it
Mouse model: Transgenic mdx mouse named mdx-Jazz
Genetic background: generated by the crossbreeding of Jazz transgenic mouse with the Dystrophin-deficient C57BL/10ScSn-DMDmdx/Jmice (mdx)
Transgene: Zinc Finger Artificial Transcription Factor ZF-ATF “Jazz”
Expression of transgene: muscle-specific
Application: Pathologic mouse model of Duchenne Muscular Dystrophy (DMD): evaluation of muscle rescue induced by ZF-ATF expression
Contacts: Claudio Passananti (IBPM): claudio.passananti@uniroma1.it
Elisabetta Mattei (IBCN): elisabetta.mattei@cnr.it
Contacts: Claudio Passananti (IBPM): claudio.passananti@uniroma1.it
Annalisa Onori (IBPM): annalisa.onori@uniroma1.it
Elisabetta Mattei (IBCN): elisabetta.mattei@cnr.it
AAV treated mice
Mouse model: WT, Dystrophin-deficient mdx and possible other appropriate pathologic mouse models.
Genetic background: WT (C57Black6), Dystrophin-deficient mdx (C57BL/10ScSn-DMDmdx)
AAV delivered gene: Zinc Finger Artificial Transcription Factors (ZF-ATFs, CNR patented), Enhanced green fluorescent protein (EGFP) and possibly any other gene
Expression of AAV delivered gene: muscle-specific and possibly any other tissue
Pathway: Autophagy - D. Trisciuoglio (daniela.trisciuoglio@uniroma1.it)
Cell Line: Human Fibrosarcoma HT1080 cells
Transgene: EGFP-LC3 or RPF- EGFP-LC3
System: CMV Promoter