• Istituto di Biologia e Patologia Molecolari


Tel :0649913980 or 0649913545
Fax :
angela.tramonti@cnr.it
ANGELA TRAMONTI
Researcher

National Research Council
Istitute of Molecular Biology and Pathology CNR
Department of Biochemical Sciences 'A. Rossi Fanelli'- Sede Distaccata, Edificio CU20- Sapienza University of Rome, P.le A. Moro 5 - 00185 Roma
  • Structure-function analyses of enzymes involved in vitamin B6 biosynthesis and salvage pathways. Studies on the molecular basis of neonatal epileptic encephalopathy.
  • Structure-function relationships and regulation of gene expression of pyridoxal-5’-phosphate-dependent transcriptional regulators in bacteria.
  • Biochemical studies on serine hydroxymethyltransferase and its role in cancer metabolic reprogramming. Identification and characterization of potential inhibitors.
  • Structure-function relationships and regulation of gene expression of pyridoxal-5’-phosphate-dependent enzymes.

Mascolo, Barile, Stufera Mecarelli, Amoroso, Merigliano, Massimi, Saggio, Hansen, Tramonti, Di Salvo, Barbetti, Contestabile, and Verni (2019) "The expression of four pyridoxal kinase (PDXK) human variants in Drosophila impacts on genome integrity"  Sci Rep. 2019 Oct 2;9(1):14188. doi: 10.1038/s41598-019-50673-4.

Barile, Tramonti, di Salvo, Nogues, Nardella, Malatesta, and Contestabile. (2019) “Allosteric feedback inhibition of pyridoxine 5'-phosphate oxidase from Escherichia coli”. J Biol Chem. 2019 Sep 4. pii: jbc.RA119.009697. doi: 10.1074/jbc.RA119.009697.

Guiducci, Paone, Tramonti, Giardina, Rinaldo, Bouzidi, Magnifico, Marani, Menendez, Fatica, Macone, Armaos, Tartaglia, Contestabile, Paiardini and Cutruzzolà (2019) “The moonlighting RNA-binding activity of cytosolic serine hydroxymethyltransferase contributes to control compartmentalization of serine metabolism” Nucleic Acid Research 47(8):4240-4254. doi: 10.1093/nar/gkz129.                                  

Tramonti, Nardella, di Salvo, Barile, Cutruzzolà, and Contestabile (2018). ”Human cytosolic and mitochondrial serine hydroxymethyltransferase isoforms in comparison: full kinetic characterization and substrate inhibition properties”  Biochemistry. 57(51):6984-6996. doi: 10.1021/acs.biochem.8b01074. 

Giardina, Paone, Tramonti, Lucchi, Marani, Magnifico, Bouzidi, Pontecorvi, Guiducci, Zamparelli, Rinaldo, Paiardini, Contestabile and Cutruzzolà (2018) “The catalytic activity of serine hydroxymethyltransferase is essential for de novo nuclear dTMP synthesis in lung cancer cells”. FEBS J. 285(17):3238-3253 . doi: 10.1111/febs.14610.

Tramonti, Paiardini, Paone, Bouzidi, Giardina, Guiducci, Magnifico, Rinaldo, McDermott, Menendez, Contestabile, and Cutruzzolà (2018) “Differential inhibitory effect of a pyrazolopyran compound on human serine hydroxymethyltransferase-amino acid complexes” Arch Biochem Biophys 653: 71-79. doi: 0.1016/j.abb.2018.07.001. 

Tramonti, Nardella di Salvo, Pascarella, and Contestabile (2018) “The MocR-like transcription factors: pyridoxal 5'-phosphate-dependent regulators of bacterial metabolism”. FEBS J. 285(21):3925-3944. doi: 10.1111/febs.14599. 

di Salvo, Mastrangelo, Nogués, Tolve, Paiardini, Carducci, Mei, Montomoli, Tramonti, Guerrini, Contestabile and Leuzzi. (2017) “Pyridoxine-5'-phosphate oxidase (Pnpo) deficiency: Clinical and biochemical alterations associated with the C.347g>A (P.·Arg116gln) mutation”. Mol Genet Metab 122, 135-42. doi: 10.1016/j.ymgme.2017.08.003.

Tramonti, Milano, Nardella, di Salvo, Pascarella, and Contestabile. (2017) “Salmonella typhimurium PtsJ is a novel MocR-like transcriptional repressor involved in regulating the vitamin B6 salvage pathway”. FEBS J. 84(3):466-484. doi: 10.1111/febs.13994.

Amidani, Tramonti, Canosa, Campanini, Maggi, Milano, di Salvo, Pascarella, Contestabile, Bettati and Rivetti. (2017) “Study of DNA binding and bending by Bacillus subtilis GabR, a PLP-dependent transcription factor” Biochim Biophys Acta - General Subjects 1861 (1 Pt A):3474-3489. doi: 10.1016/j.bbagen.2016.09.013.

Paiardini, Tramonti, Schirch, Guiducci, di Salvo, Fiascarelli, Giorgi, Maras, Cutruzzolà and Contestabile.. (2016) “Differential 3-bromopyruvate inhibition of cytosolic and mitochondrial human serine hydroxymethyltransferase isoforms, key enzymes in cancer metabolic reprogramming” Biochim Biophys Acta - Proteins and Proteomics 1864(11):1506-1517. doi: 10.1016/j.bbapap.2016.08.010.

Tramonti, Fiascarelli, Milano, di Salvo, Nogués, Pascarella, and Contestabile. (2015) “Molecular mechanism of PdxR - a transcriptional activator involved in the regulation of vitamin B6 biosynthesis in the probiotic bacterium Bacillus clausii”. FEBS J. 282(15):2966-84. doi: 10.1111/febs.13338.

Tramonti and Saliola. (2015) “Glucose 6-phosphate and alcohol dehydrogenase activities are components of dynamic macromolecular depots structures” Biochim Biophys Acta-General Subjects 1850(6): 1120-1130. doi: 10.1016/j.bbagen.2015.01.021.

Prof. Roberto Contestabile (Sapienza, Università di Roma)

Prof. Stefano Pascarella (Sapienza, Università di Roma)

Prof. Daniela De Biase (Sapienza, Università di Roma)

Prof. Francesca Cutruzzolà (Sapienza, Università di Roma)

Dott. Martino L. di Salvo (Sapienza, Università di Roma)

Dott. Michele Saliola (Sapienza, Università di Roma)

 

Education

1995: Degree cum laude in Biological Sciences, Sapienza University of Rome;

1999 Ph.D in Biochemistry, Sapienza University of Rome;

2002: Master Degree in Biotechnology, Sapienza University of Rome

 

Professional experience

1994-1995: begins her laboratory experience at Department of Biochemistry, Sapienza University of Rome, working on the expression and biochemical characterization of pyridoxal-5’-phosphate-dependent enzymes. 

1995: short visiting period at the “Institut fur Pflanzgenetik und kulturpflanzenforschung” (IPK), Gatersleben, German, supervisor Dr. Bernhard Grimm, with a grant from Human Capital and Mobility. She acquires competence in production of site-directed mutant forms of pyridoxal-5’-phosphate-dependent enzymes.

1996: grants from Sapienza University of Rome to work at the School of Molecular and Medical Biosciences (College of Cardiff, University of Wales, UK), supervisor Prof. Robert A. John. She acquires competence on enzyme kinetics.

1996-1999: during the PhD at the Department of Biochemistry (Sapienza University of Rome) she starts working on regulation of gene expression of glutamate-dependent acid resistance system in E. coli. She has broadened the expertise to the molecular biology and microbiology fields.

2000: Post-Doctoral Fellow at the Department of Biochemistry, Sapienza University of Rome, with grants from the Department of Biology of University “Roma Tre”, supervisor Prof. P. Visca

2000-2001: Post-Doctoral Fellow at the Department of Biochemistry, Sapienza University of Rome, with grants from the Faculty of Sciences.

2001-present: Researcher at the Institute of Molecular Biology and Pathology, National Research Council.

 

Peer Reviewing

Reviewer for several International Journals, including: PLoS One, BioMed Research International, Scientific reports, Plant Science, BBA-General Subject, Frontiers in Molecular Biosciences.

Reviewer of grant proposals for: PISCOPIA Fellowship Programme co-funded by Marie Curie Actions (2014) and ANR (Agence Nationale de la Reserche) France (2018).

 

National and international collaborations:

She collaborated with Profs. Robert John (School of Biosciences, Cardiff, UK), Paolo Visca (Università di Roma Tre), Vincenzo Scarlato (Università di Bologna), Dr. M. Falconi (Università di Camerino), Prof. J. Luirink (Vrije University, Olanda), Prof. C Falcone and R. Sessa (Sapienza Università di Roma).

 

Last update: January 2019