Fax :
cinzia.pisani@cnr.it
Researcher
Istitute of Molecular Biology and Pathology - National Research Council
Over the past years my research has focused on gene expression and regulation. During my PhD thesis training I focused on the characterization of proteins that directly interact with human RNA polymerase II subunits. In particular, I centered my interest on eEF1gamma/eEF1Bγ, a subunit of the holoenzyme eEF1, also known as "pancreatic tumor-related protein" and an hRPB3 interactor. My postdoctoral training extended my interests on transcription regulation to the field of zinc finger based artificial transcription factors. I’ve been actively involved in gene therapy projects based on the use of zinc finger artificial transcription factors to re-program the expression of disease-related genes, focusing on Duchenne muscular dystrophy pathology. In this field I am an inventor of two international CNR patent clusters. The study of the "regulation of gene expression" applied to biotechnologies and molecular medicine is the central thread of my work. |
-Pisani C, Strimpakos G, Gabanella F, Di Certo MG, Onori A, Severini C, Luvisetto S, Farioli-Vecchioli S, Carrozzo I, Esposito A, Canu T, Mattei E, Corbi N, Passananti C. Utrophin up-regulation by artificial transcription factors induces muscle rescue and impacts the neuromuscular junction in mdx mice. Biochim Biophys Acta Mol Basis Dis. 2018 Apr;1864(4 Pt A):1172-1182. -Pisani C, Onori A, Gabanella F, Delle Monache F, Borreca A, Ammassari-Teule M, Fanciulli M, Di Certo MG, Passananti C, Corbi N. eEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts. J Exp Clin Cancer Res. 2016; 35(1):146. -Gabanella F, Pisani C, Borreca A, Farioli-Vecchioli S, Ciotti MT, Ingegnere T, Onori A, Ammassari-Teule M, Corbi N, Canu N, Monaco L, Passananti C, Di Certo MG. SMN affects membrane remodelling and anchoring of the protein synthesis machinery. J Cell Sci. 2016; 129:804-16. -De Arcangelis V, Strimpakos G, Gabanella F, Corbi N, Luvisetto S, Magrelli A, Onori A, Passananti C, Pisani C, Rome S, Severini C, Naro F, Mattei E, Di Certo MG, Monaco L. Pathways Implicated in Tadalafil Amelioration of Duchenne Muscular Dystrophy. J Cell Physiol. 2016; 231(1):224-32. -Strimpakos G, Corbi N, Pisani C, Di Certo MG, Onori A, Luvisetto S, Severini C, Gabanella F, Monaco L, Mattei E, Passananti C. Novel adeno-associated viral vector delivering the utrophin gene regulator jazz counteracts dystrophic pathology in mdx mice. J Cell Physiol. 2014;229(9):1283-91. -Onori A, Pisani C, Strimpakos G, Monaco L, Mattei E, Passananti C, Corbi N. UtroUp is a novel six zinc finger artificial transcription factor that recognises 18 base pairs of the utrophin promoter and efficiently drives utrophin upregulation. BMC Mol Biol. 2013;14:3. -Corbi N, Batassa EM, Pisani C, Onori A, Di Certo MG, Strimpakos G, Fanciulli M, Mattei E, Passananti C. The eEF1γ subunit contacts RNA polymerase II and binds vimentin promoter region. PLoS One. 2010; 5(12):e14481. -Di Certo MG, Corbi N, Strimpakos G, Onori A, Luvisetto S, Severini C, Guglielmotti A, Batassa EM, Pisani C, Floridi A, Benassi B, Fanciulli M, Magrelli A, Mattei E, Passananti C. The artificial gene Jazz, a transcriptional regulator of utrophin, corrects the dystrophic pathology in mdx mice. Hum Mol Genet. 2010;19(5):752-60. -Poma A, Limongi T, Pisani C, Granato V, Picozzi P. Genotoxicity induced by fine urban air particulate matter in the macrophages cell line RAW 264.7. Toxicol In Vitro. 2006; 20(6): 1023-9.
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1) Gene regulatory circuits and epigenetics. 2) Differentiation and development. |
Collaborations with other institutions -Dr. Strimpakos Georgeos, IBCN-CNR, Rome, Italy. -Dr. Maurizio Fanciulli, SAFU Laboratory, Regina Elena Cancer Institute, Rome, Italy. |