• Istituto di Biologia e Patologia Molecolari


Tel :+390649255126
Fax :
annalisa.onori@uniroma1.it
ANNALISA ONORI
Research Technician

National Research Council
Istitute of Molecular Biology and Pathology CNR
Department of Molecular Medicine, Sapienza University of Rome , Viale Regina Elena 291, 00161 Rome, Italy
- Pisani C, Onori A, Gabanella F, Delle Monache F, Borreca A, Ammassari-Teule M, Fanciulli M, Di Certo MG, Passananti C, Corbi N. eEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts. J Exp Clin Cancer Res. 2016 Sep 17;35(1):146.
- Gabanella F, Pisani C, Borreca A, Farioli-Vecchioli S, Ciotti MT, Ingegnere T, Onori A, Ammassari-Teule M, Corbi N, Canu N, Monaco L, Passananti C, Di Certo MG. SMN affects membrane remodelling and anchoring of the protein synthesis machinery. J Cell Sci. 2016 Feb 15;129(4):804-16.
- De Arcangelis V, Strimpakos G, Gabanella F, Corbi N, Luvisetto S, Magrelli A, Onori A, Passananti C, Pisani C, Rome S, Severini C, Naro F, Mattei E, Di Certo MG, Monaco L. Pathways Implicated in Tadalafil Amelioration of Duchenne Muscular Dystrophy. J Cell Physiol. 2016 Jan;231(1):224-32.
- Strimpakos G, Corbi N, Pisani C, Di Certo M. G, Onori A, Luvisetto S, Severini C, Gabanella F, Monaco L, Mattei E, Passananti C. Novel adeno-associated viral vector delivering the utrophin gene regulator jazz counteracts dystrophic pathology in mdx mice. J Cell Physiol. 2014 Jan 27.
- Federman N, de la Fuente V, Zalcman G, Corbi N, Onori A, Passananti C, Romano A. Nuclear factor κB-dependent histone acetylation is specifically involved in persistent forms of memory. J Neurosci. 2013 Apr 24;33(17):7603-14.
- Onori A, Pisani C, Strimpakos G, Monaco L, Mattei E, Passananti C, Corbi N. UtroUp is a novel six zinc finger artificial transcription factor that recognises 18 base pairs of the utrophin promoter and efficiently drives utrophin upregulation. BMC Mol Biol. 2013 Jan 30;14:3. 
- Corbi N, Batassa EM, Pisani C, Onori A, Di Certo MG, Strimpakos G, Fanciulli M, Mattei E, Passananti C. The eEF1
γ subunit contacts RNA polymerase II and binds vimentin promoter region. PLoS One. 2010 Dec 31;5(12):e14481.
- Passananti C, Corbi N, Onori A, Di Certo MG, Mattei E. Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene. Methods Mol Biol. 2010;649:183-206. Book title: “Engineered Zinc finger proteins” Editors Mackay J. and Segal D.J. Springer, Human Press 2010.
- Di Certo MG, Corbi N, Strimpakos G, Onori A, Luvisetto S, Severini C, Guglielmotti A, Batassa EM, Pisani C, Floridi A, Benassi B, Fanciulli M, Magrelli A, Mattei E, Passananti C. The artificial gene Jazz, a transcriptional regulator of utrophin, corrects the dystrophic pathology in mdx mice. Hum Mol Genet. 2010 Mar 1;19(5):752-60.
- Desantis A, Onori A, Di Certo MG, Mattei E, Fanciulli M, Passananti C, Corbi N. Novel activation domain derived from Che-1 cofactor coupled with the artificial protein Jazz drives utrophin upregulation. Neuromuscul Disord. 2009 Feb;19(2):158-62. 
- Mattei E, Corbi N, Di Certo MG, Strimpakos G, Severini C, Onori A, Desantis A, Libri V, Buontempo S, Floridi A, Fanciulli M, Baban D, Davies KE, Passananti C. Utrophin up-regulation by an artificial transcription factor in transgenic mice. PLoS ONE. 2007 Aug 22;2(1):e774.
- Onori A, Desantis A, Buontempo S, Di Certo MG, Fanciulli M, Salvatori L, Passananti C, Corbi N. The artificial four zinc finger protein Bagly binds human utrophin promoter A at the endogenous chromosomal site and activates transcription. Biochem Cell Biol. 2007 Jun;85(3):358-365.
- Libri V, Onori A, Fanciulli M, Passananti C, Corbi N. The artificial zinc finger protein 'Blues' binds the enhancer of the fibroblast growth factor 4 and represses transcription. FEBS Lett. 2004 Feb 27;560(1-3):75-80.
 

Technical assistant to research activities of the group led by Dr. Claudio Passananti and Dr. Nicoletta Corbi.

Education:

1998-2002 Degree of First level of the Sanitary Professions in Technique of Biomedical Laboratory (110/110 cum laude). University of Rome “La Sapienza” Facoltà di Medicina e Chirurgia, detachment of Latina, Italy (Sanitary Professions in Technique of Biomedical Laboratory). Experimental thesis title: "Progettazione e sintesi di nuovi fattori trascrizionali zinc finger mirati a riprogrammare l’espressione di specifici geni bersaglio”. Thesis advisor: Nicoletta Corbi.

Current position and research experience:

April 2015 to present Laboratory technician at IBPM-CNR-Rome, Italy
November 2014 - March 2015 Laboratory Technician (temporary position) at IBPM-CNR-Rome, Italy
2003 – 2013 Laboratory  Graduate Technician (Co.Co.Co. contract recipient and Occasional professional services recipient) at CNR-Rome, Italy


Main research activities
Regulation of gene expression. Natural and artificial transcription factors.


Main experimental procedures:
DNA, RNA and protein isolation; DNA cloning and gene expression; Electrophoresis; Western blots; PCR and Real Time PCR; Bacterial transformation; Production of bacterial fusion proteins; Cell culture techniques; Transient and stable transfection in mammalian cells; Affinity purification of polyclonal antibodies; Immunofluorescence; Immunoprecipitation and Co-immunoprecipitation; ChIP and RNA-Chip;  Electrophoretic mobility shift assay; Production and purification of adeno-associated virus.


Practical experience in the management of biomedical equipment purchase, maintenance and technical support.