• Istituto di Biologia e Patologia Molecolari

Tel :+390649910236
Fax :+39064440062

Istitute of Molecular Biology and Pathology - National Research Council
Department of Biochemical Sciences -A. Rossi Fanelli.
Sapienza University of Rome - Edificio di Chimica Biologica Piano terra, stanza T4 P.le A. Moro 5 - 00185 Roma

My research activity is focused on the study of structure-function relationships in proteins. Structural biology represents the central part of our research together with biophysical and biochemical analysis for the characterization of biological macromolecules and their interactions. Principal research lines in which we are currently involved can be summarized as follow:

- Plant innate immunity, with particular emphasis on processes of recognition of pathogen virulence factor by host-specific molecular systems. 

- Cytochrome c biogenesis. We are interesting in characterizing proteins responsible for cytochrome c maturation in Pseudomonas aeruginosa as targets for novel antibacterial agents

- Structural and functional analysis of the human nucleolar protein nucleophosmin in relation to its role in normal karyotype acute myeloid leukemia. 

1) Chiarella, S., Federici, L., Di Matteo, A., Brunori, M., Gianni, S. The folding pathway of a functionally competent C-terminal domain of nucleophosmin: Protein stability and denatured state residual structure(2013) Biochemical and Biophysical Research Communications, . Article in Press.

 2) Chiarella, S., De Cola, A., Scaglione, G.L., Carletti, E., Graziano, V., Barcaroli, D., Lo Sterzo, C., Di Matteo, A., Di Ilio, C., Falini, B., Arcovito, A., De Laurenzi, V., Federici, L. Nucleophosmin mutations alter its nucleolar localization by impairing G-quadruplex binding at ribosomal DNA (2013) Nucleic Acids Research, 41 (5), pp. 3228-3239.

 3) Gallo, A., Lo Sterzo, C., Mori, M., Di Matteo, A., Bertini, I., Banci, L., Brunori, M., Federici, L. Structure of nucleophosmin DNA-binding domain and analysis of its complex with a G-quadruplex sequence from the c-MYC promoter(2012) Journal of Biological Chemistry, 287 (32), pp. 26539-26548. 

 4) Federici, L., Arcovito, A., Scaglione, G.L., Scaloni, F., Lo Sterzo, C., Di Matteo, A., Falini, B., Giardina, B., Brunori, M.Nucleophosmin C-terminal leukemia-associated domain interacts with G-rich quadruplex forming DNA (2010) Journal of Biological Chemistry, 285 (48), pp. 37138-37149. 

 5) Di Matteo, A., Calosci, N., Gianni, S., Jemth, P., Brunori, M., Travaglini-Allocatelli, C. Structural and functional characterization of CcmG from pseudomonas aeruginosa, a key component of the bacterial cytochrome c maturation apparatus (2010) Proteins: Structure, Function and Bioinformatics, 78 (10), pp. 2213-2221.

6) Federici, L., Lo Sterzo, C., Pezzola, S., Di Matteo, A., Scaloni, F., Federici, G., Caccuri, A.M. Structural basis for the binding of the anticancer compound 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol to human glutathione S-transferases (2009) Cancer Research, 69 (20), pp. 8025-8034. 

7) Casasoli, M., Federici, L., Spinelli, F., Matteo, A.D., Vella, N., Scaloni, F., Fernandez-Recio, J., Cervone, F., De Lorenzo, G. Integration of evolutionary and desolvation energy analysis identifies functional sites in a plant immunity protein (2009) Proceedings of the National Academy of Sciences of the United States of America, 106 (18), pp. 7666-7671. 

8) Giardina, G., Rinaldo, S., Johnson, K.A., Di Matteo, A., Brunori, M., Cutruzzolà, F. NO sensing in Pseudomonas aeruginosa: Structure of the Transcriptional Regulator DNR (2008) Journal of Molecular Biology, 378 (5), pp. 1002-1015. 

9) Di Matteo, A., Scandurra, F.M., Testa, F., Forte, E., Sarti, P., Brunori, M., Giuffrè, A. The O2-scavenging flavodiiron protein in the human parasite Giardia intestinalis (2008) Journal of Biological Chemistry, 283 (7), pp. 4061-4068. 

10) Bonivento, D., Pontiggia, D., Di Matteo, A., Fernandez-Recio, J., Salvi, G., Tsernoglou, D., Cervone, F., De Lorenzo, G., Federici, L. Crystal structure of the endopolygalacturonase from the phytopathogenic fungus Colletotrichum lupini and its interaction with polygalacturonase- inhibiting proteins (2008) Proteins: Structure, Function and Genetics, 70 (1), pp. 294-299.  

11) Di Matteo, A., Gianni, S., Schininà, M.E., Giorgi, A., Altieri, F., Calosci, N., Brunori, M., Travaglini-Allocatelli, C. A strategic protein in cytochrome c maturation: Three-dimensional structure of CcmH and binding to apocytochrome c (2007) Journal of Biological Chemistry, 282 (37), pp. 27012-27019. 

12) Federici, L., Masulli, M., Bonivento, D., Di Matteo, A., Gianni, S., Favaloro, B., Di Ilio, C., Allocati, N. Role of Ser11 in the stabilization of the structure of Ochrobactrum anthropi glutathione transferase (2007) Biochemical Journal, 403 (2), pp. 267-274. 

13) Spadoni, S., Zabotina, O., Di Matteo, A., Mikkelsen, J.D., Cervone, F., De Lorenzo, G., Mattei, B., Bellincampi, D. Polygalacturonase-inhibiting protein interacts with pectin through a binding site formed by four clustered residues of arginine and lysine (2006) Plant Physiology, 141 (2), pp. 557-564. 

14) Borgia, A., Bonivento, D., Travaglini-Allocatelli, C., Di Matteo, A., Brunori, M. Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering (2006) Journal of Biological Chemistry, 281 (14), pp. 9331-9336. 

15) Di Matteo, A., Bonivento, D., Tsernoglou, D., Federici, L., Cervone, F. Polygalacturonase-inhibiting protein (PGIP) in plant defence: A structural view (2006) Phytochemistry, 67 (6), pp. 528-533. 

16) Federici, L., Di Matteo, A., Fernandez-Recio, J., Tsernoglou, D., Cervone, F. Polygalacturonase inhibiting proteins: Players in plant innate immunity? (2006) Trends in Plant Science, 11 (2), pp. 65-70. 

17) Di Matteo, A., Giovane, A., Raiola, A., Camardella, L., Bonivento, D., De Lorenzo, G., Cervone, F., Bellincampi, D., Tsemoglou, D. Structural basis for the interaction between pectin methylesterase and a specific inhibitor protein (2005) Plant Cell, 17 (3), pp. 849-858. 

18) Travaglini-Allocatelli, C., Gianni, S., Dubey, V.K., Borgia, A., Di Matteo, A., Bonivento, D., Cutruzzolà, F., Bren, K.L., Brunori, M. An obligatory intermediate in the folding pathway of cytochrome c 552 from Hydrogenobacter thermophilus (2005) Journal of Biological Chemistry, 280 (27), pp. 25729-25734. 

 19) Cozzetto, D., Di Matteo, A., Tramontano, A. Ten years of predictions ... and counting (2005) FEBS Journal, 272 (4), pp. 881-882. 

20) Di Matteo, A., Federici, L., Mattei, B., Salvi, G., Johnson, K.A., Savino, C., De Lorenzo, G., Tsernoglou, D., Cervone, F. The crystal structure of polygalacturonase-inhibiting protein (PGIP), a leucine-rich repeat protein involved in plant defense (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (17), pp. 10124-10128. 

21) Federici, L., Caprari, C., Mattei, B., Savino, C., Di Matteo, A., De Lorenzo, G., Cervone, F., Tsernoglou, D. Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein) (2001) Proceedings of the National Academy of Sciences of the United States of America, 98 (23), pp. 13425-13430. 

1) within IBPM:

  1.a) with CNR researchers: 

Carmelinda Savino, Alessandro Giuffrè

 1.b) with IBPM associates

Maurizio Brunori (“Sapienza” University of Rome), Beatrice Vallone (“Sapienza” University of Rome)

 2) with other 

Federici Luca (University of Chieti). Carlo Travaglini-Allocatelli (“Sapienza” University of Rome). Felice Cervone (“Sapienza” University of Rome). Giulia De Lorenzo (“Sapienza” University of Rome). 




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