Fax :+39064440062
gianni.colotti@cnr.it
Senior Researcher
Istitute of Molecular Biology and Pathology - National Research Council
Department of Biochemical Sciences -A. Rossi Fanelli.
Sapienza University of Rome - Edificio di Chimica Biologica Piano terra, stanza T11 - P.le A. Moro 5 - 00185 Roma
1.Study of chemical-physical properties and of the physiopathological role of proteins and other macromolecules. |
SELECTED RECENT PUBLICATIONS (last 10 years)
1. Colotti G.*, Ilari A.
“Polyamine metabolism in Leishmania: from arginine to trypanothione”. Amino Acids. Vol. 40 (2), pp. 269-285, 2011. 2. Baiocco P., Ilari A., Ceci P., Orsini S., Gramiccia M., Di Muccio T., Colotti G.*
“Inhibitory Effect of Silver Nanoparticles on Trypanothione Reductase Activity and Leishmania infantum Proliferation.” ACS Med Chem Lett. Vol. 2 (3): 230–233, 2011. 3. Ilari A, Baiocco P, Messori L, Fiorillo A, Boffi A, Gramiccia M, Di Muccio T, Colotti G.*
“A gold-containing drug against parasitic polyamine metabolism: the X-ray structure of trypanothione reductase from Leishmania infantum in complex with auranofin reveals a dual mechanism of enzyme inhibition.” Amino Acids. Vol. 42, 2-3, 803-811, 2012.
4. Fiorillo A., Colotti G.#*, Boffi A., Baiocco P., Ilari A.
“The crystal structures of the Tryparedoxin-Tryparedoxin Peroxidase couple unveil the structural determinants of Leishmania detoxification pathway.“
PLOS Negl. Trop. Dis. Vol. 6, 8, e1781, 2012.
5. Colotti G.*, Ilari A, Boffi A, Morea V.
“Metals and Metal derivatives in Medicine.”
Mini Rev Med Chem. Vol. 13, 2, 211-221, 2013.
6. Trnkova L.; Ricci D.; Grillo C.; Colotti, G.; Altieri F.
“Green tea catechins can bind and modify ERp57/PDIA3 activity.”
Biochimica et Biophysica Acta. Vol. 1830, 3, 2671-2682, 2013.
7. Baiocco P.; Poce G.; Alfonso S.; Cocozza M.; Porretta GC.; Colotti G.*; Biava M.; Moraca F.; Botta M.; Yardley V.; Fiorillo A.; Lantella A.; Malatesta F.; Ilari A.
“Inhibition of Leishmania infantum Trypanothione Reductase by Azole-Based Compounds: a Comparative Analysis with Its Physiological Substrate by X-ray Crystallography.”
ChemMedChem. Vol. 8, 7, 1175-1183, 2013.
8. Colotti G.*, Ilari A., Fiorillo A., Baiocco P., Cinellu MA, Maiore L., Scaletti F, Gabbiani C, Messori L.
“Metal‐Based Compounds as Prospective Antileishmanial Agents: Inhibition of Trypanothione Reductase by Selected Gold Complexes.”
ChemMedChem. Vol. 8, 10, 1634-1637, 2013.
9. Colotti G.*; Baiocco P.; Fiorillo A.; Boffi A.; Poser E.; Di Chiaro F.; Ilari A.
“Structural insights into the enzymes of the trypanothione pathway: targets for antileishmaniasis drugs.”
Future Medicinal Chemistry. Vol. 5, 15, 1861-1875, 2013.
10. Lalioti VS.; Ilari A; O’Connell DJ.; Poser E.; Sandoval IV.; Colotti G.*
"Sorcin Links Calcium Signaling to Vesicle Trafficking, Regulates Polo-like Kinase 1 and is Necessary for Mitosis."
PLoS One. 2014, Jan 10; 9(1), PONE-D-13-29980R1 10.1371/journal.pone.0085438, 2014.
11. Perli E., Giordano C., Pisano A, Montanari A., Campese AF, Reyes A, Ghezzi D, Nasca A, Tuppen HA, Orlandi M, Di Micco P, Poser E, Taylor RW, Colotti G., Francisci S, Morea V, Frontali L, Zeviani M, d’Amati G.
“The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.”
EMBO Mol. Med. 2014 Feb 1; 6 (2):169-82. doi: 10.1002/emmm.201303198.
12. Sechi S., Colotti G., Belloni G., Mattei V., Frappaolo A., Raffa G.D., Fuller M.T., Giansanti M.G.
“GOLPH3 Is Essential for Contractile Ring Formation and Rab11 Localization to the Cleavage Site during Cytokinesis in Drosophila melanogaster. “
PLoS Genet. 2014 May 1;10(5):e1004305. doi: 10.1371/journal.pgen.1004305.
13. Masciarelli S, Quaranta R, Iosue I, Colotti G, Padula F, Varchi G, Fazi F, Del Rio A.
“A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4.”
ACS Chem Biol. 2014 Aug 15;9(8):1674-9. doi: 10.1021/cb500286b. Epub 2014 Jun 11. PubMed PMID: 24914804.
14. Colotti G*, Poser E, Fiorillo A, Genovese I, Chiarini V, Ilari A.
“Sorcin, a calcium binding protein involved in the multidrug resistance mechanisms in cancer cells.”
Molecules. 2014 Sep 5;19(9):13976-89. doi: 10.3390/molecules190913976. PubMed PMID: 25197934.
15. Sechi S., Frappaolo A., Belloni G., Colotti G., Giansanti MG.
“The multiple cellular functions of the oncoprotein Golgi phosphoprotein 3”
Oncotarget 2015, 6 (6), 3493.
16. Ilari A., Fiorillo A., Baiocco P., Poser E., Angiulli G., Colotti G.*
“Targeting Polyamine Metabolism for Finding New Drugs Against Leishmaniasis: A Review” Mini reviews in medicinal chemistry 2015, 15 (3), 243-252. 17. Angiulli G, Lantella A, Forte E, Angelucci F, Colotti G, Ilari A, Malatesta F.
“Leishmania infantum trypanothione reductase is a promiscuous enzyme carrying an
NADPH:O(2) oxidoreductase activity shared by glutathione reductase”.
Biochim Biophys Acta. 2015 Sep;1850(9):1891-7. doi:10.1016/j.bbagen.2015.05.022.
18. Brindisi M, Brogi S, Relitti N, Vallone A, Butini S, Gemma S, Novellino E, Colotti G, Angiulli G, Di Chiaro F, Fiorillo A, Ilari A, Campiani G.
“Structure-based discovery of the first non-covalent inhibitors of Leishmania
major tryparedoxin peroxidase by high throughput docking”.
Sci Rep. 2015 May 7;5:9705. doi: 10.1038/srep09705.
19. Ilari A, Fiorillo A, Poser E, Lalioti VS, Sundell GN, Ivarsson Y, Genovese I, Colotti G*.
“Structural basis of Sorcin-mediated calcium-dependent signal transduction”.
Sci Rep. 2015 Nov 18;5:16828. doi: 10.1038/srep16828.
20. Perli E, Fiorillo A, Giordano C, Pisano A, Montanari A, Grazioli P, Campese AF, Di Micco P, Tuppen HA, Genovese I, Poser E, Preziuso C, Taylor RW, Morea V, Colotti G*, d'Amati G.
“Short peptides from leucyl-tRNA synthetase rescue disease-causing mitochondrial tRNA point mutations”.
Hum Mol Genet. 2016 Mar 1;25(5):903-15. doi: 10.1093/hmg/ddv619.
21. Digilio, F.A., Lanati, A., Bongiovanni, A., Colotti G., Lacerra, G., Liguori, G.L.
“Quality-based model for Life Sciences research guidelines”.
Accreditation and Quality Assurance 2016; 21(3): 221-230.
22. Fracasso, G., Falvo, E., Colotti, G., (...), Arcovito, A., Ceci, P.
“Selective delivery of doxorubicin by novel stimuli-sensitive nano-ferritins overcomes tumor refractoriness”.
Journal of Controlled Release 2016; 239: 10-18.
23. Saccoliti F, Angiulli G, Pupo G, Pescatori L, Madia VN, Messore A, Colotti G, Fiorillo A, Scipione L, Gramiccia M, Di Muccio T, Di Santo R, Costi R, Ilari A.
“Inhibition of Leishmania infantum trypanothione reductase by diaryl sulfide
derivatives”.
J Enzyme Inhib Med Chem. 2017;32(1):304-310.
24. Ilari A, Fiorillo A, Genovese I, Colotti G*.
Polyamine-trypanothione pathway: an update.
Future Med Chem. 2017 Jan;9(1):61-77.
25. Poser E, Genovese I, Masciarelli S, Bellissimo T, Fazi F, Colotti G*.
Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule
Argonaute 2 Protein Binders. Methods Mol Biol. 2017;1517:223-237.
26. Bellissimo T, Masciarelli S, Poser E, Genovese I, Del Rio A, Colotti G, Fazi F.
“Small Molecules Targeting the miRNA-Binding Domain of Argonaute 2: From
Computer-Aided Molecular Design to RNA Immunoprecipitation”.
Methods Mol Biol. 2017;1517:211-221.
27. Mosca L, Falvo E, Ceci P, Poser E, Genovese I, Guarguaglini G, Colotti G*
“Use of Ferritin-Based Metal-Encapsulated Nanocarriers as Anticancer Agents”
Applied Sciences 2017, 7, 101.
28: Asteriti IA, Daidone F, Colotti G, Rinaldo S, Lavia P, Guarguaglini G, Paiardini A. “Identification of small molecule inhibitors of the Aurora-A/TPX2 complex.” Oncotarget. 2017 Mar 31. 29. Morea V, Bidollari E, Colotti G, Fiorillo A, Rosati J, De Filippis L, Squitieri F, Ilari A.
Amino Acids. 2017. doi: 10.1007/s00726-017-2417-2. “Glucose transportation in the brain and its impairment in Huntington disease: one more shade of the energetic metabolism failure?” 30: Genovese I, Fiorillo A, Ilari A, Masciarelli S, Fazi F, Colotti G*.
“Binding of doxorubicin to Sorcin impairs cell death and increases drug resistance in cancer cells”. Cell Death Dis. 2017 Jul 20;8(7):e2950. doi: 10.1038/cddis.2017.342.
31: Ferrara M, Sessa G, Fiore M, Bernard F, Asteriti IA, Cundari E, Colotti G, Ferla S, Desideri M, Buglioni S, Trisciuoglio D, Del Bufalo D, Brancale A, Degrassi F.
“Small molecules targeted to the microtubule-Hec1 interaction inhibit cancer cell growth through microtubule stabilization”. Oncogene. 2017 Sep 18. doi:10.1038/onc.2017.320.
32: Colotti G*, Fiorillo A, Ilari A.
“Metal-and metalloid-containing drugs for the treatment of trypanosomatid diseases.”
Frontiers in biosciences. 2018. 23, 954-966. doi: 10.2741/4628.
33: De Cola A, Franceschini M, Di Matteo A, Colotti G, Celani R, Clemente E, Ippoliti R, Cimini AM, Dhez AC, Vallée B, Raineri F, Cascone I, Destouches D, De Laurenzi V, Courty J, Federici L.
“N6L pseudopeptide interferes with nucleophosmin protein-protein interactions and sensitizes leukemic cells to chemotherapy.”
Cancer Letters. 2018, 412, 272-282. doi: 10.1016/j.canlet.2017.10.038
34: Falvo E, Malagrinò F, Arcovito A, Fazi F, Colotti G, Tremante E, Di Micco P, Braca A,
Opri R, Giuffrè A, Fracasso G, Ceci P.
“The presence of glutamate residues on the PAS sequence of the stimuli-sensitive nano-ferritin improves in vivo biodistribution and mitoxantrone encapsulation homogeneity.”
J Control Release. 2018 Feb 20;275:177-185. doi: 10.1016/j.jconrel.2018.02.025.
35: Ortalli M, Ilari A, Colotti G, De Ionna I, Battista T, Bisi A, Gobbi S, Rampa A, Di
Martino RMC, Gentilomi GA, Varani S, Belluti F
“Identification of chalcone-based antileishmanial agents targeting trypanothione reductase”
European journal of medicinal chemistry 152, 527-541. doi: 10.1016/j.ejmech.2018.04.057.
36. Ilari A, Genovese I, Fiorillo F, Battista T, De Ionna I, Fiorillo A, Colotti G*.
“Toward a Drug Against All Kinetoplastids: From LeishBox to Specific and Potent
Trypanothione Reductase Inhibitors.”
Mol Pharm. 2018 Jun 27. doi: 10.1021/acs.molpharmaceut.8b00185.
37. Genovese I, Ilari A, Battista T, Chiarini V, Fazi F, Fiorillo A, Colotti G.*
“Molecular bases of Sorcin-dependent resistance to chemotherapeutic agents”
Cancer Drug Resist. 2018; 1:164-180. doi:10.20517/cdr.2018.10.
38. Kahremany S, Zhenin M, Shenberger Y, Maimoun D, Colotti G, Arad M, Shainberg
A, Senderowitz H, Ruthstein S, Gruzman A.
“Peptide-based development of PKA inhibitors”
New Journal of Chemistry 2018, 42, 23:18585-18597. doi: 10.1039/C8NJ01732H.
39. Turcano L, Torrente E, Missineo A, Andreini M, Gramiccia M, Di Muccio T, Genovese
I, Fiorillo A, Harper S, Bresciani A, Colotti G*, Ilari A.
“Identification and binding mode of a novel Leishmania Trypanothione reductase
inhibitor from high throughput screening”
PLOS Neglected Tropical Diseases 2018, 12,11:e0006969.
doi:10.1371/journal.pntd.0006969
40. Pippa S, Mannironi C, Licursi V, Bombardi L, Colotti G, Cundari E, Mollica A,
Coluccia A, Naccarato V, La Regina G, Silvestri R, Negri R.
“Small Molecule Inhibitors of KDM5 Histone Demethylases Increase the
Radiosensitivity of Breast Cancer Cells Overexpressing JARID1B”
Molecules 2019, 1, 9:1739. doi:10.3390/molecules24091739.
41. Chiarini V, Tossavainen H, Sharma V, Colotti G*.
“NMR structure of a non-conjugatable, ADP-ribosylation associated, ubiquitin-like
domain from Tetrahymena thermophila polyubiquitin locus”
Biochimica et Biophysica Acta (BBA)-General Subjects 2019, 1863, 4:749. doi:
10.1016/j.bbagen.2019.01.014.
42. Colotti G*, Saccoliti F, Gramiccia M, Di Muccio T, Prakash J, Yadav S, Kumar Dubey
V, Vistoli G, Battista T, Mocci S, Fiorillo A, Bibi A, Madia VN, Messore A, Costi R, Di Santo R, Ilari A.
“Structure-guided approach to identify a novel class of anti-leishmaniasis diaryl sulfide compounds targeting the trypanothione metabolism”
Amino Acids 2019, 1-13. doi: 10.1007/s00726-019-02731-4.
43. Rinaldi T, Colotti G*.
“Use of organoids in medicinal chemistry: challenges on ethics and biosecurity”
Future Medicinal Chemistry 2019, 11 (10), 1087-1090. doi: 10.4155/fmc-2018-0341.
44. Carbo M, Brandi V, Pascarella G, Staid DS, Colotti G, Polticelli F, Ilari A, Morea V.
“Bioinformatics analysis of Ras homologue enriched in the striatum, a potential target for Huntington's disease therapy”
International journal of molecular medicine 2019, 4373. doi: 10.3892/ijmm.2019.4373.
45. Battista T, Fiorillo A, Chiarini V, Genovese I, Ilari A, Colotti G*. “Roles of Sorcin in Drug Resistance in Cancer: One Protein, Many Mechanisms, for a Novel Potential Anticancer Drug Target" Cancers (Basel). 2020 Apr 6;12(4):887. doi: 10.3390/cancers12040887 46. Caroselli S, Zwergel C, Pirolli A, Sabatino M, Xu Z, Kirsch G, Mai A, Colotti G, Altieri F, Canipari R, Valente S, Ragno R. “Discovery of the First Human Arylsulfatase A Reversible Inhibitor Impairing Mouse Oocyte Fertilization”. ACS Chem Biol. 2020 Jun 19;15(6):1349-1357. doi: 10.1021/acschembio.9b0099. 47. Genovese I, Carotti A, Ilari A, Fiorillo A, Battista T, Colotti G, Ivarsson Y. “Profiling calcium-dependent interactions between Sorcin and intrinsically disordered regions of human proteome". Biochim Biophys Acta Gen Subj. 2020 Aug;1864(8):129618. doi:10.1016/j.bbagen.2020.129618. 48. Perli E, Pisano A, Pignataro MG, Campese AF, Pelullo M, Genovese I, de Turris V, Ghelli AM, Cerbelli B, Giordano C, Colotti G, Morea V, d'Amati G. “Exogenous peptides are able to penetrate human cell and mitochondrial membranes, stabilize mitochondrial tRNA structures, and rescue severe mitochondrial defects". FASEB J. 2020 Jun;34(6):7675-7686. doi: 10.1096/fj.201903270R. 49. Battista T, Colotti G, Ilari A, Fiorillo A. “Targeting Trypanothione Reductase, a Key Enzyme in the Redox Trypanosomatid Metabolism, to Develop New Drugs against Leishmaniasis and Trypanosomiases”. Molecules. 2020 Apr 21;25(8):1924. doi: 10.3390/molecules25081924. 50. Colotti G*, Rinaldi T. “The central role of gut microbiota in drug metabolism and personalized medicine”. Future Med Chem. 2020 May 29. doi: 10.4155/fmc-2020-0023. 51. Turcano L, Battista T, De Haro ET, Missineo A, Alli C, Paonessa G, Colotti G, Harper S, Fiorillo A, Ilari A, Bresciani A. “Spiro-containing derivatives show antiparasitic activity against Trypanosoma brucei through inhibition of the trypanothione reductase enzyme”. PLoS Negl Trop Dis. 2020 May 21;14(5):e0008339. doi: 10.1371/journal.pntd.0008339. 52. Mascia A, Cirafici AM, Bongiovanni A, Colotti G, Lacerra G, Di Carlo M, Digilio FA, Liguori GL, Lanati A, Kisslinger A. “A failure mode and effect analysis (FMEA)-based approach for risk assessment of scientific processes in non-regulated research laboratories. Accreditation And Quality Assurance. 2020. doi: 10.1007/s00769-020-01441-9. 53. Genovese I, Giamogante F, Barazzuol L, Battista T, Fiorillo A, Vicario M, D'Alessandro G, Cipriani R, Limatola C, Rossi D, Sorrentino V, Poser E, Mosca L, Squitieri F, Perluigi M, Arena A, van Petegem F, Tito C, Fazi F, Giorgi C, Calì T, Ilari A, Colotti G*. “Sorcin is an early marker of neurodegeneration, Ca2+ dysregulation and endoplasmic reticulum stress associated to neurodegenerative diseases.” Cell Death Dis. 2020 Oct 15;11(10):861. doi: 10.1038/s41419-020-03063-y. 54. Flego M, Colotti G, Ascione A, Dupuis ML, Petrucci E, Riccioni R, Andreotti M, Raggi C, Boe A, Barca S, Gellini M, Vella S, Mallano A. “Isolation and preliminary characterization of a human 'phage display'-derived antibody against neural adhesion molecule-1 antigen interfering with fibroblast growth factor receptor-1 binding.” Hum Antibodies. 2020 Oct 23. doi: 10.3233/HAB-200431. 55. Mosca L, Ilari A, Fazi F, Assaraf YG, Colotti G*. “Taxanes in cancer treatment: Activity, chemoresistance and its overcoming.” Drug Resist Updat. 2021 Jan;54:100742. doi: 10.1016/j.drup.2020.100742. 56. Chiarini V, Fiorillo A, Camerini S, Crescenzi M, Nakamura S, Battista T, Guidoni L, Colotti G*, Ilari A. “Structural basis of ubiquitination mediated by protein splicing in early Eukarya.” Biochim Biophys Acta Gen Subj. 2021 May;1865(5):129844. doi: 10.1016/j.bbagen.2021.129844. 57. Battista T, Pascarella G, Staid DS, Colotti G, Rosati J, Fiorillo A, Casamassa A, Vescovi AL, Giabbai B, Semrau MS, Fanelli S, Storici P, Squitieri F, Morea V, Ilari A. “Known Drugs Identified by Structure-Based Virtual Screening Are Able to Bind Sigma-1 Receptor and Increase Growth of Huntington Disease Patient-Derived Cells.” Int J Mol Sci. 2021 Jan 28;22(3):1293. doi: 10.3390/ijms22031293. 58. Colotti G, Failla CM, Lacal PM, Ungarelli M, Ruffini F, Di Micco P, Orecchia A, Morea V. "Neuropilin-1 is required for endothelial cell adhesion to soluble vascular endothelial growth factor receptor 1". FEBS Journal 2021, 10.1111. https://doi.org/10.1111/febs.16119 59. Valentini E, DAguanno S, Di Martile M, Montesano C, Ferraresi V, Patsilinakos A, Sabatino M, Antonini L, Valente S, Mai A, Colotti G, Ragno R, Trisciuoglio D, Del Bufalo D. "Targeting the anti-apoptotic Bcl-2 family proteins: machine learning virtual screening and biological evaluation of new small molecules". Theranostics 2021; doi:10.7150/thno.64233; accepted 60. Fiorillo A, Morea V., Colotti G., Ilari A. "Huntingtin ubiquitination mechanisms and novel possible therapies to decrease the toxic effects of mutated Huntingtin". Journal of Personalized Medicine, 2021, 11, 1309. doi: 10.3390/jpm11121309.
|
Dr. Andrea Ilari |
Prof. Godfrey L. Smith, University of Glasgow (Scotland, UK) |
DEGREES: 1989: degree in Biological Sciences (with honors), University of Rome "La Sapienza", Italy. PROFESSIONAL EXPERIENCE: 1990-1993: Ph. D. Dept. Biochemical Sciences, University Sapienza, Rome, Italy TEACHING 1997-2010: Course of Molecular Biology, School of Specialization in Pathological Anatomy, Faculty of Medicine, University "Sapienza", Rome, Italy.
Italian Substitute, COST (European Cooperation in Science and Technology) Harmonizing standardisation strategies to increase efficiency and competitiveness of European life-science research (CHARME). Since 2009: Member of NetwOrK, the Network for Outreach and Knowledge CNR |