• Istituto di Biologia e Patologia Molecolari

stocks and engineered cell lines

Collections, engineered stocks and cell lines @IBPM



IBPM is a partner of Addgene (https://www.addgene.org), a non-profit plasmid repository that allows deposit and retrieval of plasmids and other vectors.

 
Human cell line collection


Pathway: RNA processing
- E. Caffarelli (elisa.caffarelli@uniroma1.it)
Cell line: Human Neuroblastoma SH-SY5Y cells
Transgene: shRNA-TDP43
System: tet on (inducible loss of function)
 
Pathway: neuronal differentiation, apoptosis - E. Caffarelli (elisa.caffarelli@uniroma1.it)
Cell line: Human Neuroblastoma SKNBE cells
Transgene: Linc-NeD125 lncRNA
System: tet on (inducible gain of function)
 
Pathway: myc pathway  - B. Illi. (barbara.illi@uniroma1.it)
Cell line: HEK293T cells, glioblastoma U87MG cells and glioblastoma stem cells
Transgene: FlagOmomyc expressing lentiviral vector
System: tet on (inducible gain of function)
 
Cell line: HEK293T and glioblastoma stem cells 
Transgene: Myc shRNA expressing lentiviral vector .
System: tet on 
 
Pathway: centrosomes  - G.Guarguaglini (giulia.guarguaglini@uniroma1.it)
Cell line: human epithelial cells immortalized with hTERT (hTERT-RPE1)
Transgene: Aurora-A and/or TPX2
system: tet on (inducible overexpression of full length and deletion mutants)
 
 
Pathway: kinetochores  - F. Degrassi (francesca.degrassi@uniroma1.it)
Cell line: HeLa human cervical cancer cells
Transgene: Hec1
system: tet on (inducible expression of N-ter GFP-tagged Hec1 or C-ter GFP-tagged Hec1) 
 
Pathways:  nuclear transport; mitosis  -  P. Lavia (patrizia.lavia@uniroma1.it)
Cell line: HeLa human cervical cancer cells
Transgene: Importin beta-1 (KPNB-1) or Chromosome Mantainance-1/Exportin-1 (CRM1/XPO1)
System: tet on (inducible expression of EGFP-tagged Importin beta-1 or EGFP-tagged CRM1)
 
 
Pathway: autophagy - D. Trisciuoglio (daniela.trisciuoglio@uniroma1.it)
Cell line: Human fibrosarcoma HT1080  cells
Transgene: eGFP-LC3 or RPF- eGFP-LC3
System: CMV promoter 
 

Budding yeast mutant strains - Saccharomyces cerevisiae 
Contact Filetici P.  patrizia.filetici@uniroma1.it
 
 
> 500 strains of budding yeast Saccharomyces cerevisiae  
 
genetic background,  
W303   ade2-1, trp1-1, leu2-3, 112 his3-11,15, ura3, can1-100, ssd1.
 
Collection of mutants, disrupted and tagged strains in epigenetic modulators. e.g.   
- acetylatrnsferases, 
- ubiquitin proteases, 
- individual subunits of multiproteic chromatin remodeling complex (SAGAc). 
The collection also features double and triple disrupted strains for genetic analysis and epistatic assays. 
 
Disrupted strains: KAT-Gcn5, DUB-Ubp8, Sgf73, Sus1, Ada2, Ada3, Spt7, Spt20
tagging: Gcn5-Myc, Ubp8-Myc, Gcn5-GFP, Cse4-myc, 
strains expressing His6-Ub for the biochemical purification of Ub-proteins.
TS mutants for kinetochore components, mitotic checkpoint and microtubule-binding proteins, associated to GCN5 or UBP8 disruptions.

 
Plants - Arabidopsis thaliana

 
Loss- and gain-of-function HD-Zip II transcription factor mutants
HD-Zip II constitutive and inducible overexpressing lines
HA-, GFP- and YFP-tagged HD-Zip II proteins
Combinations of mutants belonging to different HD-Zip protein families (HD-Zip II, HD-Zip III)
Root, shoot and flower markers introgressed in loss-of-function HD-Zip II mutants
HD-Zip II markers introgressed in root, shoot and flower regulatory mutants
Combinations of HD-Zip II mutants and root, shoot and flower regulatory mutants
Auxin markers introgressed in loss-of-function HD-Zip II mutants
Combinations of HD-Zip II mutants and auxin transport/signalling mutants
 
 
Light signalling mutants                                                  
Auxin perception mutants                                                                                   
Auxin transport mutants                                                        
Auxin conjugate synthetase mutants
Loss-of-function ARF (auxin-response factor) mutants
ARF inducible overexpressing lines
PIN and ABCB (auxin transport) inducible overexpressing lines
DR5:GUS introgressed into the wild type and auxin perception mutant backgrounds 
DR5:GFP introgressed into the wild type and auxin trasnport mutant backgrounds 
 
Mouse model collection
 
Transgenic mice
Mouse model: Transgenic mouse named Tg-Jazz (CNR patented)
Genetic background: generated on the Hybrid genetic background BDF1 (C57Black6 x DBA)
Transgene: Zinc Finger Artificial Transcription Factor ZF-ATF “Jazz” (GenBank accession number AJ243577.1.)
Expression: muscle-specific
 
Contacts: Claudio Passananti (IBPM): claudio.passananti@uniroma1.it
Elisabetta Mattei (IBCN): elisabetta.mattei@cnr.it
 
Mouse model: Transgenic mdx mouse named mdx-Jazz
Genetic background: generated by the crossbreeding of Jazz transgenic mouse with the Dystrophin-deficient C57BL/10ScSn-DMDmdx/Jmice (mdx)
Transgene: Zinc Finger Artificial Transcription Factor ZF-ATF “Jazz” 
Expression of transgene: muscle-specific
Application: Pathologic mouse model of Duchenne Muscular Dystrophy (DMD): evaluation of muscle rescue induced by ZF-ATF expression
 
Contacts: Claudio Passananti (IBPM): claudio.passananti@uniroma1.it
Elisabetta Mattei (IBCN): elisabetta.mattei@cnr.it
 
 
Contacts: Claudio Passananti (IBPM): claudio.passananti@uniroma1.it
                  Annalisa Onori (IBPM): annalisa.onori@uniroma1.it
                  Elisabetta Mattei (IBCN): elisabetta.mattei@cnr.it
 
AAV treated mice
Mouse model: WT, Dystrophin-deficient mdx and possible other appropriate pathologic mouse models.
Genetic background: WT (C57Black6), Dystrophin-deficient mdx (C57BL/10ScSn-DMDmdx) 
AAV delivered gene: Zinc Finger Artificial Transcription Factors (ZF-ATFs, CNR patented), Enhanced green fluorescent protein (EGFP) and possibly any other gene
Expression of AAV delivered gene: muscle-specific and possibly any other tissue
 
Pathway: Autophagy - D. Trisciuoglio (daniela.trisciuoglio@uniroma1.it)
Cell Line: Human Fibrosarcoma HT1080 cells
Transgene: EGFP-LC3 or RPF- EGFP-LC3
System: CMV Promoter