• Istituto di Biologia e Patologia Molecolari


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alice.pajoro@cnr.it
ALICE PAJORO
Researcher

National Research Council
Istitute of Molecular Biology and Pathology CNR
Sapienza University of Rome - Piazzale Aldo Moro 5, 00185 - Rome, Italy - Department of Biology and Biotechnologies C.Darwin - Building CU026 (General Physiology, ex BCS)
My research projects focus on molecular mechanisms that regulate plant development in response to endogenous and environmental signals during the reproductive phase.

Current projects:

StemFlow:  In plants, the transition from vegetative to reproductive growth requires coordinated developmental changes in different tissues. How the initiation of flower development at the apex of the plant is coordinated with alterations in stem growth to support the inflorescence is poorly understood. Key regulators of floral transition exhibit precise cellular expression patterns in stems, in the project we study how their expression patterns in stems and shoot apices are coordinated and define their functions in controlling stem growth.
Funded by the Max-Planck Partnership. In collaboration with Prof. G. Coupland at Max-Planck Institute for Plant Breeding Research, Cologne, Gemany

Top of the Crops: INTERAZIONI TRA LUCE E ORMONI NEL CONTROLLO DELL’APERTURA FIORALE IN SPECIE MODELLO E ORTIVE. L’apertura del fiore rappresenta l’ultimo stadio dello sviluppo fiorale ed è controllato da fattori ormonali che ambientali, la cui interazione definisce il successo e la durata del processo. Questo tratto impatta su molte colture ortive Brassicaceae, tra cui una tipica coltura laziale, la cima di rapa, in quanto una prematura apertura del fiore ne comporta un deprezzamento. Il progetto si articola su due fasi: l’identificazione dei geni responsabili dell’apertura dei fiori nella specie modello Arabidopsis thaliana che è anch’essa una Brassicacea e il successivo trasferimento della conoscenza alla coltura ortiva cime di rapa.
Funded by Regione Lazio. In collaboration with Prof. G. Serino Dipartimento di Biologia e Biotecnologie C. Darwin, La Sapienza,  & Dr D. Giannino, Istituto dei Sistemi Biologici, CNR, Roma, Italy

EcoRap: Valorizzazione della collezione del germoplasma di cime di rapa pugliesi in un’ottica di economia sostenibile.
Il progetto propone di agire su due fronti per rendere la coltura della cima di rapa più produttiva e sostenibile, da un lato individuare con approccio genomico le varianti strutturali della biodiversità delle cime associate al ritardo nell’antesi, dall’altro esplorare l’uso dei prodotti di scarto come fonte di composti micostatici e/o micotossici nei confronti di funghi. Nell’ottica di un’economia circolare i residui della coltura potranno essere convertiti in prodotto ad alto valore aggiunto, contribuendo così alla riduzione dei rifiuti e ai costi di smaltimento a carico degli agricoltori.
Funded by Progetto Bio-Memory, Dipartimento di Scienze Bio-Agroalimentari
In collaboration with Dr. Alessandra Ricelli, CNR-IBPM

Functional Genomics Approaches for Improving Plant Inflorescence Architecture: Sufficient food production is threatened by the fast-growing world population and global climate change. To produce sufficient food for future generations in a sustainable manner, it is necessary to develop innovative strategies for plant production. This can only be achieved through an increased fundamental knowledge on how plants develop. This project intends to contribute to increase plant yield by studying pathways that control inflorescence development.
Funded by MUR, PRIN2020. In collaboration with Prof M. Kater, Universita' degli Studi di Milano, Italy

Rucol-ITA: Rucola is a food with high nutritional value because is reach of micronutrients and antioxidants. This characteristic has increased the consumer request of rucola in the European Market. Italy represent one of the major producers of Rucola in Europe. The project aims to select of new rucola varieties with higher performance.
In collaboration with Prof. L. Faino at Dipartimento di Biologia Ambientale, La Sapienza, Roma, Italy
  • Severing E, Faino L, Jamge S, Busscher M, Kuijer-Zhang Y, Bellinazzo F, Angenent GC, Immink RGH, Pajoro A "Arabidopsis thaliana ambient temperature responsive lncRNAs and their role in flowering time control." (2018) BMC Plant Biology 18:145. DOI: 10.1186/s12870-018-1362-x
  • Pajoro A, Muiño JM, Angenent GC, Kaufmann K "Profiling nucleosome occupancy by MNase-seq: Experimental protocol and computational analysis" (2018) Methods in Molecular Biology, 1675, pp.167181. DOI:10.1007/978-1-4939-7318-7_11
  • Pajoro A, Severing E, Angenent GC, Immink RGH. (2017) “Histone H3 lysine 36 methylation affects temperature-induced alternative splicing and flowering in plants” Genome Biology 18:102 DOI: 10.1186/s13059-017-1235-x
  • González-Grandío E, Pajoro A, Franco-Zorrilla JM, Tarancón C, Immink RGH, Cubas P (2016) “Abscisic acid signaling is controlled by a BRANCHED1/HD-ZIP I cascade in Arabidopsis axillary budsProceedings of the National Academy of Sciences of the United States of America (PNAS) 114:245-254 DOI: 10.1073/pnas.1613199114
  • Pajoro A, Verhage L, Immink RGH. (2016) “Plasticity versus Adaptation of Ambient-Temperature Flowering ResponseTrends in Plant Science 21 (1): 6-8. DOI:10.1016/j.tplants.2015.11.015
  • Muino JM, De Bruijn S, Pajoro A, Geuten K, Vingron M, Angenent GC, Kaufmann K. (2016) “Evolution of DNA-binding sites of a floral master regulatory transcription factorMolecular Biology and Evolution 33 (1): 185-200 DOI:10.1093/molbev/msv210
  • Capovilla G, Pajoro A, Immink RGH, Schmid M. (2015) “Role of alternative pre-mRNA splicing in temperature signalingCurrent Opinion in Plant Biology 27: 97-103 DOI:10.1016/j.pbi.2015.06.016
  • Pajoro A, Biewers S, Dougali E, Leal Valentim F, Mendes MA, Porri A, Coupland G, Van de Peer Y, van Dijk ADJ, Colombo L, Davies B and Angenent GC (2014) “The (r)evolution of gene regulatory networks controlling Arabidopsis plant reproduction, a two decades history.” Journal of Experimental Botany 65 (17): 4731-4745 DOI:10.1093/jxb/eru233
  • Pajoro A, Madrigal P, Muiño JM, Matus JT, Jin J, Mecchia MA, Debernardi JM, Palatnik JF, Balazadeh S, Arif M, Ó’Maoiléidigh DS, Wellmer F, Krajewski P, Riechmann JL, Angenent GC, Kaufmann K (2014) “Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development.” Genome Biology 15:R41 DOI:10.1186/gb-2014-15-3-r41
  • Dreni L, Erreni S, Caporali E, Pajoro A, Pilatone A, Yun D, Zhang D, Kater MM (2011) Functional analysis of all AGAMOUS subfamily members in rice reveals their roles in reproductive organ identity determination and meristem determinacy.” Plant Cell 23, 2850-2863 DOI: 10.1105/tpc.111.087007
  • Kaufmann K, Pajoro A, Angenent GC (2010) “Regulation of transcription in plants: mechanisms controlling developmental switches.” Nature Reviews Genetics 11, 830–842. DOI: 10.1038/nrg2885
Dr. Maura Cardarelli, CNR-IBPM
Prof. G. Coupland at Max-Planck Institute for Plant Breeding Research, Cologne, Gemany
Prof. G. Serino Dipartimento di Biologia e Biotecnologie C. Darwin, La Sapienza
Dr D. Giannino, Istituto dei Sistemi Biologici, CNR, Roma, Italy
Prof M. Kater, Universita' degli Studi di Milano, Italy
Prof. L. Faino at Dipartimento di Biologia Ambientale, La Sapienza, Roma, Italy
Prof. R. Immink, Wageningen University, The Netherlands

2019-present: Researcher/Junior PI Istituto di Biologia a patologia molecolari (IBPM) Consiglio Nazionale delle Ricerche c/o Dipartimento di Biologia e Biotecnologie “Charles Darwin”. 

2018 – 2020:   EMBO long-term Post Doc Fellow
in Prof G. Coupland’s group, Max Plank Institute for Plant Breeding, Cologne, Germany. The research focuses on the coordination of floral transition and stem development

2014 – 2017          Post Doc in Prof R. Immink’s group, Plant Research International, Wageningen, The Netherlands. The research focuses on understand the impact of ambient temperature in regulation of developmental processes in Arabidopsis, within the ERA-CAPS network, FLOWPLAST.

2009 – 2014          PhD in Molecular Biology in Prof. G. Angenent’s department supervised by Dr. K. Kaufmann, Wageningen University, Wageningen, The Netherlands. My project was carried out in the framework of the Marie Curie ITN project SYSFLO. The project focused on the gene regulatory networks underlying flower development in Arabidopsis.

2008 – 2009          Intern at the Universitá degli Studi di Milano in Prof. M. Kater’s group. My project focused on the role of OsMADS13 and OsMADS21 in rice ovule and kernel development and the functional conservation of gene function between Arabidopsis and rice.

2006                       Intern at the Universitá degli Studi di Milano in Prof. G. Gavazzi and Prof G. Consonni’s group. The project focused on the characterization fused leaves mutant (fdl) in Zea mais