• Istituto di Biologia e Patologia Molecolari


Tel :+390649910548
Fax :+39064440062
stefano.gianni@uniroma1.it
STEFANO GIANNI
Full Professor

Istitute of Molecular Biology and Pathology - National Research Council
Department of Biochemical Sciences -A. Rossi Fanelli.
Sapienza University of Rome - Edificio di Chimica Biologica Piano terra, stanza T12 P.le A. Moro 5 - 00185 Roma

My research interest lies in understanding the fundamental aspects of protein folding, dynamics and disorder.

 

1) Haq SR, Chi CN, Bach A, Dogan J, Engström A, Hultqvist G, Karlsson A, Lundström P, Montemiglio LC, Strømgaard K, Gianni S, Jemth P. (2012) Side chain interactions form late and cooperatively in the binding reaction between disordered peptides and PDZ domains. J. Am. Chem. Soc. 134, 599-605.

 

2) Gianni, S., Ivarsson, Y., De Simone, A., Travaglini-Allocatelli, C., Brunori, M., Vendruscolo M., (2010) Characterization of the Structure of a Misfolded Intermediate Populated during the Folding Process of a PDZ domain Nature Struct. Mol Biol. 17, 1431-1437.

 

3) Scaloni, F., Federici, L., Brunori, M., Gianni, S., (2010) Deciphering the folding transition state structure and denatured state properties of nucleophosmin C-terminal domain. Proc. Natl. Acad. Sci, USA 107, 5447-5452.

 

4) Ivarsson, Y., Travaglini-Allocatelli, C., Brunori, M., Gianni, S., (2009) Engineered symmetric connectivity highlights malleability in protein folding pathways J. Am. Chem. Soc. 131, 11727- 11733.

 

5) Travaglini-Allocatelli, C., Ivarsson, Y., Jemth, P., and Gianni, S., (2009) Folding and stability of globular proteins and implications for function. Curr. Opin. Struct. Biol. 19, 3-7.

 

6) Ivarsson, Y, Travaglini-Allocatelli, C., Brunori, M., Gianni, S., (2008) Folding and Misfolding in a naturally circularly permuted PDZ domain. J. Biol. Chem. 283, 8954-8960.

 

7) Ivarsson, Y., Travaglini-Allocatelli, C., Jemth, P., Malatesta, F., Brunori, M. and Gianni, S. (2007) An on-pathway intermediate in the folding of a PDZ domain. J Biol Chem. 282, 8568-8572

 

8) Gianni, S., Geeierhaas, C.D., Calosci, N., Jemth, P., Vuister, G.W., Travaglini-Allocatelli, C., Vendruscolo, M. and Brunori M. (2007) A PDZ domain recapitulates a unifying mechanism for protein folding. Proc. Natl. Acad. Sci USA, 104, 128-133.

 

9) Gianni, S., Walma, T., Arcovito, A., Calosci, N., Bellelli, A., Engstrom, Å, Travaglini- Allocatelli, C., Brunori, M., Jemth, P. and Vuister, G.W. (2006) Demonstration of long-range interactions in a PDZ domain by NMR, kinetics, and protein engineering. Structure, 14, 1801- 1809.

 

10) Gianni, S., Guydosh, N.R., Khan, F., Caldas, T.D., Mayor, U., White, G.W.N., DeMarco, M.L., Daggett, V. & Fersht A.R. (2003) Unifying features in protein folding mechanisms. Proc. Natl. Acad. Sci USA, 100, 13286-13291.

Angela Morrone, Rajanish Giri

 

 

 

 

1) Chi, C.N, Engstrom, Å, Gianni, S., Larsson, M. and Jemth, P. (2006) Two conserved residues govern the salt and pH dependencies of the binding reaction of a PDZ domain. J.Biol. Chem., 281, 36811-36888.

I.F.: 5,328

 

2) Gianni, S., Walma, T., Arcovito, A., Calosci, N., Bellelli, A., Engstrom, Å, Travaglini-Allocatelli, C., Brunori, M., Jemth, P. and Vuister, G.W. (2006) Demonstration of long-range interactions in a PDZ domain by NMR, kinetics, and protein engineering. Structure, 14, 1801-1809.

I.F.: 6,337

 

3) Gianni, S., Geeierhaas, C.D., Calosci, N., Jemth, P., Vuister, G.W., Travaglini-Allocatelli, C., Vendruscolo, M. and Brunori M. (2007) A PDZ domain recapitulates a unifying mechanism for protein folding. Proc. Natl. Acad. Sci USA, 104, 128-133.

I.F.: 9,771

 

4) Federici, L., Masulli, M., Bonivento, D., Di Matteo, A., Gianni, S., Favaloro, B., Di Ilio, C. and Allocati, N., (2007) Role of Ser11 in the stabilization of the structure of Ochrobactrum anthropi glutathione transferase. Biochem J. 2007 403, 267-274

I.F.: 5,016

 

5) Ivarsson, Y., Travaglini-Allocatelli, C., Jemth, P., Malatesta, F., Brunori, M. and Gianni, S.Ý (2007) An on-pathway intermediate in the folding of a PDZ domain.

J Biol Chem. 282, 8568-8572

I.F.: 5,328

 

6) Chi, C.N*, Gianni, S.*Ý, Calosci, N., Travaglini-Allocatelli, C. Engstrom, Å, and Jemth, P. (2007) A conserved folding mechanism for PDZ domains. FEBS Letters 2007 581, 1109-1113.

I.F.: 3,601

 

7) Gianni, S.Ý, Ivarsson, Y., Jemth, P., Brunori, M., Travaglini-Allocatelli, C. (2007) Identification and characterization of protein folding intermediates. Biophys. Chem 128, 105-113.

I.F.: 2,108

 

8) Gianni, S., Brunori, M., Travaglini-Allocatelli, C., (2007) Plasticity of the protein folding landscape: switching between on and off-pathway intermediates  Arch. Biochem. Biophys. 466 172-176.

I.F.: 3,022

 

9) Di Matteo, A., Gianni, S., Schininà, M.E., Giorgi, A., Altieri, F., Calosci, N., Brunori, M., Travaglini-Allocatelli, C. (2007) A strategic protein in cytochrome c maturation: 3D structure of CcmH and binding to apocytochrome c. J. Biol. Chem. 282, 27012-27019.

I.F.: 5,328

 

10) Jemth, P., Gianni, S.Ý (2007) PDZ domains: folding and binding. Biochemistry 46, 8701-8708

I.F.: 3,226

 

11) Gianni, S.Ý, Ivarsson, Y., Bah, A., Bush-Pelic, L.A., Di Cera, E. (2007) Mechanism of Na(+) binding to thrombin resolved by ultra-rapid kinetics. Biophys. Chem. 131, 111-114.

I.F.: 2,108

 

12) Ivarsson, Y., Travaglini-Allocatelli, C., Morea, V., Brunori, M., Gianni, S.Ý (2008) The folding pathway of an engineered circularly permuted PDZ domain. Protein Eng. Des. Sel 21 155-160.

I.F.: 3,023

 

13) Ivarsson, Y., Travaglini-Allocatelli, C., Brunori, M., Gianni, S.Ý (2008) Mechanisms of proten folding. Eur. Biophys. J. 37, 721-728

I.F.: 2,387

 

14) Jemth, P., Johnson, C.M., Gianni, S., Fersht, A.R. (2008) Demonstration by burst phase analysis of a robust folding intermediate in the FF domain Protein Eng. Des. Sel. 21, 207-214.

I.F.: 3,023

 

15) Ivarsson, Y, Travaglini-Allocatelli, C., Brunori, M., Gianni, S.Ý, (2008) Folding and Misfolding in a naturally circularly permuted PDZ domain. J. Biol. Chem. 283, 8954-8960.

I.F.: 5,328

 

16) Borgia, A., Gianni, S., Brunori, M., Travaglini-Allocatelli, C., (2008)Fast folding kinetics and stabilization of apo-cytochrome c FEBS Letters .582 1003-1007.

I.F.: 3,601

17) Parrini, C., Bemporad, F., Baroncelli, A., Gianni, S., Travaglini-Allocatelli, C., Kohn, J.E., Ramazzotti, M., Chiti, F., Taddei, N., (2008) The folding process of acylphosphatase from Escherichia coli is remarkably accelerated by the presence of a disulfide bond. J. Mol. Biol. 379, 1107-1118.

I.F.: 4,008

 

18) Calosci, N., Chi, C.N., Richter, B., Camilloni, C., Engstrom, A, Eklund, L, Travaglini-Allocatelli, C., Gianni, S.Ý, Vendruscolo, M.Ý, Jemth, P.Ý, (2008) Comparison of successive transition states for folding reveals alternative early folding pathways of two homologous proteins Proc. Natl. Acad. Sci. USA, 105, 19241-19246.

I.F.: 9,771

 

19) Sicorello, A., Torrassa, S., Soldi, G., Gianni, S., Travaglini-Allocatelli, C., Taddei, N., Relini, A., Chiti, F., (2009) Agitation and high ionic strength induce amyloidogenesis of a folded PDZ domain in native conditions Biophys. J 96, 2289-2298.

I.F.: 4,218

 

20) Travaglini-Allocatelli, C., Ivarsson, Y., Jemth, P., and Gianni, S.,Ý (2009) Folding and stability of globular proteins and implications for function. Curr. Opin. Struct. Biol. 19, 3-7.

I.F.: 9,903

 

21) Scaloni, F., Gianni, S., Federici, L., Falini, B., Brunori, M. (2009) Folding mechanism of the C-terminal domain of nucleophosmin: residual structure in the denatured state and its pathophysiological significance. FASEB J. 23, 2360-2365.

I.F.: 6,515

 

22) Federici, L., Masulli, M., Gianni, S., Di Ilio, C., Allocati, N. (2009) A conserved hydrogen-bond network stabilizes the structure of Beta class glutathione S-transferases. Biochem Biophys Res Commun. 382: 525-529.

I.F.: 2,595

 

23) Ivarsson, Y., Travaglini-Allocatelli, C., Brunori, M., Gianni, S., (2009) Engineered symmetric connectivity highlights malleability in protein folding pathways J. Am. Chem. Soc. 131, 11727-11733.

I.F.: 9,023

 

24) Chi, C.N., Bach, A., Engström, A., Wang, H., Strømgaard, K., Gianni, S.Ý, Jemth, P. Ý (2009) A sequential binding mechanism in a PDZ domain. Biochemistry. 48, 7089-7097.

I.F.: 3,226

 

25) Savino, C., Montemiglio, L.C., Sciara, G., Miele, A.E., Kendrew, S.G., Jemth, P., Gianni, S.Ý, Vallone, B., (2009) Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate. J. Biol. Chem. 284, 29170-29179.

I.F.: 5,328

 

26) Gianni, S., Brunori, M., Jemth, P., Oliveberg, M., Zhang, M., (2009) Distinguishing between smooth and rough free energy barriers in protein folding. Biochemistry 48, 11825-11830.

I.F.: 3,226

 

27) Giansanti, F., Di Leandro, L., Koutris, I., Pitari, G., Fabbrini, M.S., Lombardi, A., Flavell, D.J., Flavell, S.U., Gianni, S. Ý, Ippoliti, R. (2010) Engineering a switchable toxin: the potential use of PDZ domains in the expression, targeting and activation of modified saporin variants. Protein Eng. Des, Sel. 23. 61-68.

I.F.: 3,023

 

28) Scaloni, F., Federici, L., Brunori, M., Gianni, S., (2010) Deciphering the folding transition state structure and denatured state properties of nucleophosmin C-terminal domain. Proc. Natl. Acad. Sci, USA 107, 5447-5452.

I.F.: 9,771

 

29) Haq, S.R., Jurgens, M.C., Chi, C.N., Koh, C.S., Elfstrom, L., Selmer, M., Gianni, SÝ, Jemth, P (2010) The plastic energy landscape of protein folding: a triangular folding mechanism with an equilibrium intermediate for a small protein domain. J Biol Chem. 285, 18051-18059

I.F.: 5,328

 

30) Di Matteo, A., Calosci, N., Gianni ,S., Jemth, P., Brunori, M., Travaglini-Allocatelli, C. (2010) Structural and functional characterization of CcmG from Pseudomonas aeruginosa, a key component of the bacterial cytochrome c maturation apparatus. Proteins. Struct. Funct. Bioinf.  78, 2213-21.

I.F.: 2,813

 

31) Montemiglio, L.C., Gianni, S., Vallone, B., Savino, C. (2010) Azole drugs trap cytochrome P450 EryK in alternative conformational states. Biochemistry 49, 9199-9206.

I.F.: 3,226

 

32) Gianni, S., Ivarsson, Y., De Simone, A., Travaglini-Allocatelli, C., Brunori, M., Vendruscolo M., (2010) Characterization of the Structure of a Misfolded Intermediate Populated during the Folding Process of a PDZ domain Nature Struct. Mol Biol. 17, 1431-1437.

I.F.: 13,685

 

33) Morrone, A., McCully, M.E., Bryan, P.N., Brunori, M., Daggett, V., Gianni, S.Ý, Travaglini-Allocatelli, C., (2011) The denatured state dictates the topology of two proteins with almost identical sequence but different native structure and function. J. Biol., Chem. . 286, 3863-3872.

I.F.: 5,328

 

34) Gianni, S., Haq, S.R., Montemiglio, L.C., Jürgens, M.C., Engström, Å., Chi, C.N., Brunori, M., Jemth, P. (2011) Sequence-specific long range networks in PSD-95/discs large/ZO-1 (PDZ) domains tune their binding selectivity. J. Biol. Chem. 286, 27167-27175.

I.F.: 5,328

 

35) Morrone, A., Giri, R., Toofanny, R.D., Travaglini-Allocatelli, C., Brunori, M., Daggett, V., Gianni, S.Ý, (2011) GB1 is not a two-state folder: identification and characterization of an on-pathway intermadiate. Biophys. J. in press.

I.F.: 4,218

 

36) Giardina, G., Montioli, R., Gianni, S., Cellini, B., Paiardini, A., Borri-Voltattorni, C., Cutruzzolà, F., (2011) The open conformation of human DOPA decarboxylase reveals the mechanism of PLP addition to Group II decarboxylases. Proc. Natl. Acad. Sci. USA 5 Dec 2011 [Epub ahead of print].

I.F.: 9,771

 

37) Haq SR, Chi CN, Bach A, Dogan J, Engström A, Hultqvist G, Karlsson A, Lundström P, Montemiglio LC, Strømgaard K, Gianni S, Jemth P. (2011) Side chain interactions form late and cooperatively in the binding reaction between disordered peptides and PDZ domains. J. Am. Chem. Soc. 30 Nov. 2011 [Epub ahead of print]

I.F.: 9,023

 

38) Brunori, M., Gianni, S., Giri, R., Morrone, A., Travaglini-Allocatelli, C., (2012) Morphogenesis of a protein: folding pathways and the energy landscape Bioch. Soc. Trans 40(2):429-32.

I.F.: 3,989

 

39) Hultqvist G, Pedersen SW, Chi CN, Strømgaard K, Gianni S, Jemth P. (2012) An expanded view of the protein folding landscape of PDZ domains. Biochem Biophys Res Commun. 421, 550-553.

I.F.: 2,595

 

40) Chi, C.N., Bach, A., Stromgaard, K., Gianni, S., Jemth, P., (2012) Ligand binding by PDZ domains. Biofactors, in press.

I.F.: 2,793

 

41) Giri., R., Morrone, A., Travaglini-Allocatelli C., Jemth, P., Brunori, M., Gianni, S.Ý, (2012) The folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc. Natl. Acad. Sci. USA. In press

I.F.: 9,771

 

 

•Maurizio Brunori (Sapienza, University of Rome)

 

•Per Jemth (Uppsala University)

 

•Valerie Daggett (Seattle University)

 

•Gilad Haran (Weizmann Institute of Science)

 

•Michele Vendruscolo (University of Cambridge)

Born in Rome, Italy, 27th January 1976

 

Education:

 

Degree, in Biological Sciences, 110/110 cum laude, 14 December 1999. University of Rome “La Sapienza”.

PhD in Biochemistry, 27 March 2003. Title: Snapshots of Protein folding. The folding pathway of cytochrome c551 from Pseudomonas Aeruginosa. University of Rome “La Sapienza”.

 

Appointments:

 

Assistant Professor, (from 30 December 2005) Institute of Molecular Biology and Pathology, CNR, Rome, Italy. 

Postdoctoral fellow, (1 June 2005 - 28 December 2005) founded by the Istituto Pasteur- Fondazione Cenci Bolognetti at the Department of Biochemical Sciences, University of Rome “La Sapienza”.

Research Assistant, (1 March 2004 - 31 May 2005) at the Department of Biochemical Sciences, University of Rome “La Sapienza”. 

Visiting Scientist, (1 October 2001 – 31 December 2003) Centre for Protein Engineering, Medical Research Council, Cambridge, UK.

 

Editorial activities:

 

Editorial Board Member of Protein Engineering Design & Selection (Oxford Journals) 

Editorial Board Member of Research Letters in Biochemistry (Hindawi) 

Editorial Board Member of Open Journal of Biochemistry (Ross Science Publishers)

 

Reviewer for several journals including: Proc. Natl. Acad. Sci. USA, J. Am. Chem. Soc., J. Mol. Biol., Biochemistry, Biochem. J., Protein Sci., PLOS Computational Biology, Proteins Struct. Funct. Bioinf., Prot. Eng. Des. Sel., Biophys. Chem.,

 

Awards:

 

-May 2009 Accademia Nazionale delle Scienze, detta dei XL, e Accademia Nazionale dei Lincei. “Vincenzo Caglioti" International Prize for Chemistry.

-September 2010 Young Researcher Prize, CNR Department of Life Sciences.

 

Qualifications:

Qualification as Associate Professor in the disciplinary sector BIO/10 Biochemistry (held in July 2010)