ANNA BARILE
EDUCATION
2016-2019: PhD Degree in Biochemistry, Sapienza University, Roma.
Thesis project: “Characterisation of the key enzymes involved in vitamin B6 salvage pathway in Escherichia coli and humans”
2012-2015: Master’s Degree in Biology and Human Evolution, Tor Vergata University, Roma.
Thesis project: “Study of the effect of selenite on the expression of Ctr1 and on the susceptibility to cisplatin in different cancer cell lines”
2009-2012: Bachelor’s Degree in Biology, Tor Vergata University, Roma.
Thesis project: “Evaluation of copper content in cells overexpressing Glutaredoxin-1 by atomic absorption spectrophotometry”
POST-LAUREAM EXPERIENCE
November 2019 – December 2019: Research fellow, Istitute Pasteur Italy-Fondazione Cenci Bolognetti, c/o Department of Biochemical Sciences, Sapienza University, Roma.
Project: “Regulation of vitamin B6 metabolism and bioavailability in Eubacteria”.
PUBLICATIONS
1. Elisa Mascolo and Anna Barile, Lorenzo Stufera Mecarelli, Noemi Amoroso, Chiara Merigliano, Arianna Massimi, Isabella Saggio, Torben Hansen, Angela Tramonti, Martino L. Di Salvo, Fabrizio Barbetti, Roberto Contestabile and Fiammetta Vernì. “The expression of four pyridoxal kinase (PDXK) human variants in Drosophila impacts on genome integrity”. October 2, 2019, Scientific Reports.
2. Anna Barile, Angela Tramonti, Martino L. di Salvo, Isabel Nogués, Caterina Nardella, Francesco Malatesta and Roberto Contestabile. “Allosteric feedback inhibition of pyridoxine 5’-phosphate oxidase from Escherichia coli”. September 4, 2019, Journal of Biological Chemistry.
3. Anastasia De Luca, Anna Barile, Mario Arciello, Luisa Rossi. “Copper homeostasis as target of both consolidated and innovative strategies of anti-tumor therapy”. July 13, 2019, Journal of Trace Elements in Medicine and Biology.
4. Caterina Nardella, Dalila Boi, Martino L. di Salvo, Anna Barile, Jörg Stetefeld, Angela Tramonti and Roberto Contestabile. “Isolation of a complex formed beteween Acinetobacter baumannii HemA and HemL, key enzymes of tetrapyrroles biosynthesis”. February 26, 2019, Frontiers in Molecular Biosciences.
5. Angela Tramonti, Caterina Nardella, Martino L. di Salvo, Anna Barile, Francesca Cutruzzolà and Roberto Contestabile. “Human cytosolic and mitochondrial serine hydroxymethyltransferase isoforms in comparison: full kinetic characterisation and substrate inhibition properties”. November 30, 2018, Biochemistry.
KEYWORDS Vitamin B6 metabolism, Pyridoxal 5’-phosphate salvage pathway, neonatal epileptic encephalopathy
2016-2019: PhD Degree in Biochemistry, Sapienza University, Roma.
Thesis project: “Characterisation of the key enzymes involved in vitamin B6 salvage pathway in Escherichia coli and humans”
2012-2015: Master’s Degree in Biology and Human Evolution, Tor Vergata University, Roma.
Thesis project: “Study of the effect of selenite on the expression of Ctr1 and on the susceptibility to cisplatin in different cancer cell lines”
2009-2012: Bachelor’s Degree in Biology, Tor Vergata University, Roma.
Thesis project: “Evaluation of copper content in cells overexpressing Glutaredoxin-1 by atomic absorption spectrophotometry”
POST-LAUREAM EXPERIENCE
November 2019 – December 2019: Research fellow, Istitute Pasteur Italy-Fondazione Cenci Bolognetti, c/o Department of Biochemical Sciences, Sapienza University, Roma.
Project: “Regulation of vitamin B6 metabolism and bioavailability in Eubacteria”.
PUBLICATIONS
1. Elisa Mascolo and Anna Barile, Lorenzo Stufera Mecarelli, Noemi Amoroso, Chiara Merigliano, Arianna Massimi, Isabella Saggio, Torben Hansen, Angela Tramonti, Martino L. Di Salvo, Fabrizio Barbetti, Roberto Contestabile and Fiammetta Vernì. “The expression of four pyridoxal kinase (PDXK) human variants in Drosophila impacts on genome integrity”. October 2, 2019, Scientific Reports.
2. Anna Barile, Angela Tramonti, Martino L. di Salvo, Isabel Nogués, Caterina Nardella, Francesco Malatesta and Roberto Contestabile. “Allosteric feedback inhibition of pyridoxine 5’-phosphate oxidase from Escherichia coli”. September 4, 2019, Journal of Biological Chemistry.
3. Anastasia De Luca, Anna Barile, Mario Arciello, Luisa Rossi. “Copper homeostasis as target of both consolidated and innovative strategies of anti-tumor therapy”. July 13, 2019, Journal of Trace Elements in Medicine and Biology.
4. Caterina Nardella, Dalila Boi, Martino L. di Salvo, Anna Barile, Jörg Stetefeld, Angela Tramonti and Roberto Contestabile. “Isolation of a complex formed beteween Acinetobacter baumannii HemA and HemL, key enzymes of tetrapyrroles biosynthesis”. February 26, 2019, Frontiers in Molecular Biosciences.
5. Angela Tramonti, Caterina Nardella, Martino L. di Salvo, Anna Barile, Francesca Cutruzzolà and Roberto Contestabile. “Human cytosolic and mitochondrial serine hydroxymethyltransferase isoforms in comparison: full kinetic characterisation and substrate inhibition properties”. November 30, 2018, Biochemistry.
KEYWORDS Vitamin B6 metabolism, Pyridoxal 5’-phosphate salvage pathway, neonatal epileptic encephalopathy