Fax :
martino.disalvo@uniroma1.it
Assistant Professor
Istitute of Molecular Biology and Pathology - National Research Council
Department of Biochemical Sciences - A. Rossi Fanelli.
| My research activity within IBPM concerns a research project lead by Dr Angela Tramonti entitled "Molecular characterization of enzymes involved in vitamin B1- and B6-dependent epilepsies", in collaboration with Victoria Bunik from the Belozersky Research Insitute of Lomonosov Moscow State University (Moscow, Russia). In this context, our interest is focused on the mechanism of vitamin B6 homeostasis in humans and on the molecular basis of vitamin B6-dependend epilepsy, with particular attention on missense mutations of pyridoxine 5'-phosphate oxidase. We are also collaborating with clinicians to characterise novel mutations and correlate medical symptoms to the biochemical profile of patients. |
LIST OF PUBLICATIONS (Dr. Martino Luigi Di Salvo)
Pubblicazioni “peer-reviewed” su riviste scientifiche internazionali
1. Tramonti A, Nardella C, di Salvo ML, Barile A, Cutruzzolà F, Contestabile R (2018) Human cytosolic and mitochondrial serine hydroxymethyltransferase isoforms in comparison: full kinetic characterization and substrate inhibition properties. Biochemistry. doi: 10.1021/acs.biochem.8b01074. [Epub ahead of print]
2. Tramonti A, Nardella C, di Salvo ML, Pascarella S, Contestabile R (2018) The MocR-like transcription factors: pyridoxal 5'-phosphate-dependent regulators of bacterial metabolism. FEBS J, 285, 3925-3944. doi: 10.1111/febs.14599. Epub 2018 Jul 5. PMID:29974999
3. Ma Y, Di Salvo ML, Budisa N (2018) Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins. Methods Mol Biol, 1728, 127-135; doi: 10.1007/978-1-4939-7574-7_7.
4. Martino L. di Salvo, Mario Mastrangelo, Isabel Nogués, Manuela Tolve, Alessandro Paiardini, Carla Carducci, Davide Mei, Martino Montomoli, Angela Tramonti, Renzo Guerrini, Roberto Contestabile, Vincenzo Leuzzi (2017) Biochemical Data from the Characterization of a New Pathogenic Mutation uf Human Pyridoxine-5'-Phosphate Oxidase (PNPO). Data in Brief, 15, 868-875; doi.org/10.1016/j.dib.2017.10.032 [Available online 28 October 2017].
5. di Salvo ML, Mastrangelo M, Nogués I, Tolve M, Paiardini A, Carducci C, Mei D, Montomoli M, Tramonti A, Guerrini R, Contestabile R, Leuzzi V (2017) Pyridoxine-5'-phosphate oxidase (PNPO) deficiency: Clinical and biochemical alterations associated with the C.347g>A (P.·Arg116gln) mutation. Mol Genet Metab, 122, 135-142; doi: 10.1016/j.ymgme.2017.08.003.
6. Tramonti A, Milano T, Nardella C, di Salvo ML, Pascarella S, Contestabile R. (2017) Salmonella typhimurium PtsJ is a novel MocR-like transcriptional repressor involved in regulating the vitamin B6 salvage pathway. FEBS J, 284, 466-484; doi: 10.1111/febs.13994. Epub 2017 Jan 9.
7. Amidani D, Tramonti A, Canosa AV, Campanini B, Maggi S, Milano T, di Salvo ML, Pascarella S, Contestabile R, Bettati S, Rivetti C. (2016) Study of DNA binding and bending by Bacillus subtilis GabR, a PLP-dependent transcription factor. Biochim Biophys Acta, 1861(1 Pt A):3474-3489; doi: 10.1016/j.bbagen.2016.09.013. Epub 2016 Sep 14.
8. Angelaccio S, Milano T, Tramonti A, Di Salvo ML, Contestabile R, Pascarella S. (2016) Data from computational analysis of the peptide linkers in the MocR bacterial transcriptional regulators. Data Brief, 9, 292-313; doi: 10.1016/j.dib.2016.08.064. eCollection 2016 Dec.
9. Milano T, Angelaccio S, Tramonti A, Di Salvo ML, Contestabile R, Pascarella S (2016) Structural properties of the linkers connecting the N- and C- terminal domains in the MocR bacterial transcriptional regulators. Biochimie Open, 3, 8–18.
10. Paiardini A, Tramonti A, Schirch D, Guiducci G, di Salvo ML, Fiascarelli A, Giorgi A, Maras B, Cutruzzolà F, Contestabile R. (2016) Differential 3-bromopyruvate inhibition of cytosolic and mitochondrial human serine hydroxymethyltransferase isoforms, key enzymes in cancer metabolic reprogramming. Biochim Biophys Acta, 1864, 1506-17; doi:10.1016/j.bbapap.2016.08.010. Epub 2016 Aug 13.
11. Milano T, Angelaccio S, Tramonti A, Di Salvo ML, Contestabile R, Pascarella S. (2016) A Bioinformatics Analysis Reveals a Group of MocR Bacterial Transcriptional Regulators Linked to a Family of Genes Coding for Membrane Proteins. Biochem Res Int, 2016:4360285; doi: 10.1155/2016/4360285. Epub 2016 Jun 30.
12. Mkrtchyan G, Aleshin V, Parkhomenko Y, Kaehne T, Di Salvo ML, Parroni A, Contestabile R, Vovk A, Bettendorff L, Bunik V (2015) Molecular mechanisms of the non-coenzyme action of thiamin in brain: biochemical, structural and pathway analysis. Sci Rep, 5, 12583; doi: 10.1038/srep12583.
13. Tramonti A, Fiascarelli A, Milano T, di Salvo ML, Nogués I, Pascarella S, Contestabile R (2015) Molecular mechanism of PdxR - a transcriptional activator involved in the regulation of vitamin B6 biosynthesis in the probiotic bacterium Bacillus clausii. FEBS J, 282, 2966-84; doi: 10.1111/febs.13338.
14. Milano T, Di Salvo ML, Angelaccio S, Pascarella S (2015) Conserved water molecules in bacterial serine hydroxymethyltransferases. Protein Eng Des Sel, 28, 415-26; doi: 10.1093/protein/gzv026. Epub 2015 May 18.
15. di Salvo ML, Nogués I, Parroni A, Tramonti A, Milano T, Pascarella S, Contestabile R (2015) On the mechanism of Escherichia coli pyridoxal kinase inhibition by pyridoxal and pyridoxal 5'-phosphate. Biochim Biophys Acta, 1854,1160-6; doi: 10.1016/j.bbapap.2015.01.013.
16. Giardina G, Brunotti P, Fiascarelli A, Cicalini A, Costa MG, Buckle AM, di Salvo ML, Giorgi A, Marani M, Paone A, Rinaldo S, Paiardini A, Contestabile R, Cutruzzolà F (2015) How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state. FEBS J, 282, 1225-41; doi: 10.1111/febs.13211.
17. Remesh SG, Ghatge MS, Ahmed MH, Musayev FN, Gandhi A, Chowdhury N, di Salvo ML, Kellogg GE, Contestabile R, Schirch V, Safo MK (2015) Molecular basis of E. coli L-threonine aldolase catalytic inactivation at low pH. Biochim Biophys Acta, 1854, 278-83; doi: 10.1016/j.bbapap.2014.12.023.
18. Elsinghorst PW, di Salvo ML, Parroni A, Contestabile R (2015) Inhibition of human pyridoxal kinase by 2-acetyl-4-((1R,2S,3R)-1,2,3,4-tetrahydroxybutyl)imidazole (THI). J Enzyme Inhib Med Chem, 30, 336-40; doi: 10.3109/14756366.2014.915396.
19. Angelaccio, S. , Di Salvo, M.L., Parroni, A., Di Bello, A., Contestabile, R., Pascarella, S. (2014) Structural stability of cold-adapted serine hydroxymethyltransferase, a tool for β-hydroxy-α-amino acid biosynthesis. Journal of Molecular Catalysis B: Enzymatic, 110, 171-177; doi:10.1016/j.molcatb.2014.10.007.
20. Ying Ma, Hernán Biava, Roberto Contestabile, Nediljko Budisa and Martino Luigi di Salvo (2014) Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins, Molecules, 19, 1004-1022; doi:10.3390/molecules19011004.
21. Martino L. di Salvo, Soumya G. Remesh, Mirella Vivoli, Mohini S. Ghatge, Alessandro Paiardini, Simona D’Aguanno, Martin K. Safo and Roberto Contestabile (2014) On the catalytic mechanism and stereospecificity of Escherichia coli L-threonine aldolase, FEBS J, 281, 129-145; doi: 10.1111/febs.12581.
22. Martino Luigi di Salvo, Neel Scarsdale, Galina Kazanina, Roberto Contestabile, Verne Schirch, and H. Tonie Wright (2013) Structure-Based Mechanism for Early PLP-Mediated Steps of Rabbit Cytosolic Serine Hydroxymethyltransferase Reaction, BioMed Research International, 2013:458571; dx.doi.org/10.1155/2013/458571.
23. Barbara Campanini, Stefano Bettati, Martino Luigi di Salvo, Andrea Mozzarelli, and Roberto Contestabile (2013) Asymmetry of the Active Site Loop Conformation between Subunits of Glutamate-1-semialdehyde Aminomutase in Solution. BioMed Research International, 2013:353270; dx.doi.org/10.1155/2013/353270.
24. di Salvo ML, Contestabile R, Paiardini A, Maras B (2013) Glycine consumption and mitochondrial serine hydroxymethyltransferase in cancer cells: the heme connection. Med Hypotheses, 80, 633-6; doi: 10.1016/j.mehy.2013.02.008. Epub 2013 Mar 6.
25. Martino L. di Salvo, Rita Florio, Alessandro Paiardini, Mirella Vivoli, Simona D’Aguanno and Roberto Contestabile (2013) Alanine Racemase from Tolypocladium Inflatum: A Key PLP-dependent Enzyme in Cyclosporin Biosynthesis and a Model of Catalytic Promiscuity. Arch Biochem Biophys, 529, 55-65; doi: 10.1016/j.abb.2012.11.011. Epub 2012 Dec 3.
26. Amit K. Gandhi, Jigar V. Desai, Mohini S. Ghatge, Martino L. di Salvo, Stefano Di Biase, Richmond Danso-Danquah, Faik N. Musayev, Roberto Contestabile, Verne Schirch, Martin K. Safo (2012) Crystal Structures of Human Pyridoxal Kinase in Complex with the Neurotoxins, Ginkgotoxin and Theophylline: Insights into Pyridoxal Kinase Inhibition. PloS ONE, 7(7):e40954. Epub 2012 Jul 18.
27. Mohini S. Ghatge, Roberto Contestabile, Martino L. di Salvo, Jigar V. Desai, Amit K. Gandhi, Christina M. Camara, Rita Florio, Isabel N González, Alessia Parroni, Verne Schirch and Martin K. Safo (2012) Pyridoxal 5’-Phosphate is a Slow Tight Binding Inhibitor of E. coli Pyridoxal Kinase. PloS ONE, 7(7):e41680. Epub 2012 Jul 25.
28. Martino L. di Salvo, Martin K. Safo, Roberto Contestabile (2012) The vitamin B6 salvage pathway: biomedical aspects of pyridoxal 5’-phosphate availability. Front Biosci (Elite Ed), 4, 897-913.
29. Daidone F, Florio R, Rinaldo S, Contestabile R, di Salvo ML, Cutruzzolà F, Bossa F, Paiardini A. (2011) In silico and in vitro validation of serine hydroxymethyltransferase as a chemotherapeutic target of the antifolate drug pemetrexed. Eur J Med Chem, 46, 1616-21.
30. di Salvo ML, Contestabile R, Safo MK. (2011) Vitamin B(6) salvage enzymes: Mechanism, structure and regulation. Biochim Biophys Acta, 1814, 1597-608.
31. Florio R, di Salvo ML, Vivoli M, Contestabile R. (2011) Serine hydroxymethyltransferase: A model enzyme for mechanistic, structural, and evolutionary studies. Biochim Biophys Acta, 1814, 1489-96.
32. Wilkins BJ , Marionni S, Young DD, Liu J, Wang Y, Di Salvo ML, Deiters A, Cropp TA. (2010) Site-specific incorporation of fluorotyrosines into proteins in Escherichia coli by photochemical disguise. Biochemistry,Biochemistry." Biochemistry. 49, 1557-9.
33. Vivoli M, Angelucci F, Ilari A, Morea V, Angelaccio S, di Salvo ML, Contestabile R. (2009) Role of a conserved active site cation-pi interaction in Escherichia coli serine hydroxymethyltransferase. Biochemistry, 48, 12034-46.
34. Musayev FN, Di Salvo ML, Saavedra MA, Contestabile R, Ghatge MS, Haynes A, Schirch V, Safo MK. (2009) Molecular basis of reduced pyridoxine 5'-phosphate oxidase catalytic activity in neonatal epileptic encephalopathy disorder. J Biol Chem, 284, 30949-56.
35. Gandhi AK, Ghatge MS, Musayev FN, Sease A, Aboagye SO, di Salvo ML, Schirch V, Safo MK (2009) Kinetic and structural studies of the role of the active site residue Asp235 of human pyridoxal kinase. Biochem Biophys Res Commun, 381, 12-5.
36. Amadasi A, Bertoldi M, Contestabile R, Bettati S, Cellini B, Di Salvo M., Borri-Voltattorni C, Bossa F, Mozzarelli A (2007) Pyridoxal 5'-phosphate enzymes as targets for therapeutic agents. Curr Med Chem, 14, 1291-1324.
37. Musayev FN, Di Salvo M, Ko TP, Gandhi AK, Goswami A, Schirch V, Safo MK (2007) Crystal Structure of human pyridoxal kinase: Structural basis of M+ and M2+ activation. Protein Sci, 16, 2184-2194.
38. Safo MK, Musayev FN, Di Salvo M, Hunt S, Claude J-B, Schirch V (2006) Crystal Structure of Pyridoxal Kinase from the Escherichia coli pdxK Gene: Implications for the Classification of Pyridoxal Kinases. J Bacteriol, 188, 4542-4552.
39. Safo MK, Musayev FN, Hunt S, di Salvo M, Scarsdale N, and Schirch V (2004) Crystal Structure of the PdxY Protein from Escherichia coli. J Bacteriol, 186, 8074-8082.
40. di Salvo ML, Hunt S, Schirch V (2004) Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases. Protein Expr Purif, 36, 300-306.
41. Szebenyi DM, Musayev FN, di Salvo ML, Safo MK, Schirch V (2004) Serine hydroxymethyltransferase: role of glu75 and evidence that serine is cleaved by a retroaldol mechanism. Biochemistry, 43, 6865-6876.
42. Musayev FN, Di Salvo ML, Ko TP, Schirch V, Safo MK (2003) Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase. Protein Sci, 12, 1455-1463.
43. di Salvo ML, Safo MK, Musayev FN, Bossa F, Schirch V (2003) Structure and mechanism of Escherichia coli pyridoxine 5'-phosphate oxidase. Biochim Biophys Acta, 1647, 76-82.
44. Bertoldi M, Cellini B, Paiardini A, Di Salvo M, Borri Voltattorni C (2003) Treponema denticola cystalysin exhibits significant alanine racemase activity accompanied by transamination: mechanistic implications. Biochem J, 371, 473-483.
45. di Salvo ML, Ko T-P, Musayev FN, Raboni S, Schirch V, Safo MK (2002) Active Site Structure and Stereospecificity of Escherichia coli Pyridoxine 5’-phopshate Oxidase. J Mol Biol, 315, 385-397.
46. Schirch V, Delle Fratte S, di Salvo M (2002) Detection of isoAspartate Residues as Post-Translational Modification of Protein and Peptides, in Methods Mol Biol 19, 269-276.
47. Contestabile R, Paiardini A, Pascarella S, di Salvo M, D’Aguanno S, Bossa F (2001) L-Threonine aldolase, serine hydroxymethyltransferase and fungal alanine racemase. Eur J Biochem, 268, 1-18.
48. Fu TF, di Salvo M, Schirch V (2001) Distribution of B6 Vitamers in Escherichia coli as Determined by Enzymatic Assay. Anal Biochem, 298, 314-321.
49. Safo MK, Musayev FN, di Salvo ML, Schirch V (2001) X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with pyridoxal 5'-phosphate at 2.0 Å resolution. J Mol Biol, 310, 817-26.
50. Fu TF, di Salvo M, Schirch V (2001) Enzymatic determination of homocysteine in cell extracts. Anal Biochem, 290, 359-65.
51. Safo MK, Mathews I, Musayev FN, Di Salvo M, Thiel DJ, Abraham DJ, Schirch V (2000) X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with FMN at 1.8 Å resolution. Structure, 8, 751-762.
52. Di Salvo ML, Delle Fratte S, Maras B, Wright HT, Schirch V (1999) Deamidation of Asparagine Residues in a Recombinant Serine Hydroxymethyltrasferase. Arch Biochem Biophys, 372, 271-279.
53. Ko T, Safo MK, Musayev FN, Di Salvo M, Wang C, Wu S, Abraham DJ (1999) Crystal Structure of Human Erythrocyte Catalase. Acta Crystallogr D Biol Crystallogr, 56, 241-245.
54. Musayev FN, Safo MK, Di Salvo M, Schirch V, Abraham DJ (1999) Crystallization and Preliminary X-ray Crystallographic Analysis of Pyridoxine 5-Phosphate Oxidase Complexed with Flavin Mononucleotide. J Struct Biol, 127, 88-91.
55. Di Salvo M, Delle Fratte S, De Biase D, Bossa F, Schirch V (1998) Purification and Characterization of Recombinant Rabbit Cytosolic Serine Hydroxymethyltransferase. Protein Expr Purif, 13, 177-183.
56. Di Salvo M, Yang E, Zhao G, Winkler ME, Schirch V (1998) Expression, Purification, and Characterization of Recombinant Escherichia coli Pyridoxine 5'-Phosphate Oxidase. Protein Expr Purif, 13, 249-256.
57. Pascarella S, Angelaccio S, Contestabile R, Delle Fratte S, Di Salvo M, Bossa F (1998) The Structure of Serine Hydroxymethyltransferase as modelled by Homology and validated by Site Directed Mutagenesis. Protein Sci, 7, 1976-1982.
58. Kruschwitz H, Ren S, Di Salvo M, Schirch V (1995) Expression, Purification and Characterization of Human Cytosolic Serine Hydroxymethyltransferase. Protein Expr Purif, 6, 411-416.
Capitoli di libri scientifici 1. Martino L. di Salvo, Nediljko Budisa, and Roberto Contestabile (2013) PLP‐dependent Enzymes: a Powerful Tool for Metabolic Synthesis of Non‐canonical Amino Acids, in Molecular evolution and control (Molekulare Entwicklung und Kontrolle), Beilstein Bozen Symposium on Molecular Engineering and Control, May 14th – 18th 2012, Prien (Chiemsee), Germany. Received: 5th June 2013 / Published: 13th December 2013.
2. Mohini S. Ghatge, Martino L. Di Salvo, Roberto Contestabile, Dorothy N. Eseonu, Sayali Karve, Verne Schirch and Martin K. Safo (2012) Molecular Defects of Vitamin B6 Metabolism Associated with Neonatal Epileptic Encephalopathy, in Miscellanea on Encephalopathies - A Second Look, ISBN: 978-953-51-0558-9, DOI: 10.5772/29149.
3. Di Salvo M, Yang E, Safo M, Musayev F, Schirch V (2000) Structure and Function of E. coli Pyridoxine Phosphate Oxidase. In: Iriarte A, Kagan HM, Martinez-Carrion M, Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins. BASEL-BOSTON-BERLIN.
4. Schirch V, Di Salvo M, Scarsdale N, Delle Fratte S, Wright HT (2000) 3-Dimensional Structures of Rabbit Cytosolic and E. coli Serine Hydroxymethyltransferase. In: Iriarte A, Kagan HM, Martinez-Carrion M, Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins. BASEL-BOSTON-BERLIN.
Libri di testo universitari Autore e curatore: 1. “Metodologie biochimiche” – 2012; A cura di Maria Carmela Bonaccorsi, Roberto Contestabile, Martino Di Salvo. Casa Editrice Ambrosiana. ISBN 9788808183293
Traduttore e curatore della versione italiana dei seguenti testi: 2. Appling, Anthony-Cahill, Mathews “Biochimica. Molecole e metabolismo”. Ediz. mylab. Con eText. Con aggiornamento online. Curatori: M. Bolognesi, R. Contestabile, M. L. Di Salvo. Editore: Pearson 2017. ISBN 9788891902740 3. Garret-Grisham “Biochimica” – 2014 Piccin, Padova, Italy. ISBN: 9788829922338 4. Muller-Estler “Biochimica - per studenti di scienze della vita”- 2007 Idelson-Gnocchi, Napoli, Italy. ISBN: 9788879474627 5. Garret-Grisham “Principi di Biochimica – con messa a fuoco su quella umana” – 2004 Piccin, Padova, Italy. ISBN: 9788829916931
Presentazioni orali a convegni nazionali e internazionali 1. “Semisynthetic production of unnatural amino acids and their direct incorporation into peptides and proteins” Convegno annuale del Dipartimento di Biologia e Biotecnologie “Charles Darwin”, Facoltà di Scienze Matematiche Fisiche e Naturali, Sapienza Università di Roma. Abbazia di Sant’Andrea in Flumine, Ponzano Romano, Italy, 9-10 giugno 2015.
2. “Protein engineering on the PLP-dependent enzyme threonine aldolase: genetic/metabolic screening through cell growth requirements”, Biokatalyse Seminars, Institüt für Chemie - Technische Universität Berlin, Germany, November 11, 2012
3. “The Vitamin B6 Salvage Pathway: Biomedical Aspects of Pyridoxal 5’-Phosphate Availability” The Third International Conference on Cofactors 03 (ICC-03). University of Turku, Turku, Finland, July 10-15, 2011
4. “Structural and functional studies on E. coli and human pyridoxal kinases” 1st International Interdisciplinary Conference on Vitamins, Coenzymes, and Biofactors 2005, Awaji Island, Hyogo, Japan, Nov. 2005.
5. “Structure and mechanism of Escherichia coli pyridoxine 5’-phosphate oxidase”, in the 3rd International Symposium on Vitamin B6, PQQ, Carbonyl Catalysis and Quinoproteins, Southampton, United Kingdom, 14-19 April 2002.
6. “Structure and function of pyridoxine-5’-phosphate oxidase”, in the 2nd International Symposium on Vitamin B6, PQQ, Carbonyl Catalysis and Quinoproteins, Santa Fe, New Mexico, U.S.A., 31 October- 5 November 1999.
7. “Identificazione di siti di deamidazione come ipotetici segnali di invecchiamento nella serina idrossimetiltrasferasi” at “9a riunione Nazionale dei Dottorandi di Ricerca in Discipline Biochimiche”, Brallo di Pregola (PV), Italy, 10-13 June 1996. |
Martino Luigi di Salvo si è laureato nel 1994 in Scienze Biologiche presso la Facoltà di Scienze Matematiche, Fisiche e Naturali dell’Università La Sapienza di Roma. Presso la stessa Università ha conseguito il Dottorato di Ricerca in Biochimica nel 1998. Dopo un periodo di ricerca all’estero presso il Department of Biochemistry and Molecular Biophysics della Virginia Commonwealth University USA, in qualità di Post-doctoral Fellow, nel 2001 ha preso servizio presso l’Università di Roma La Sapienza come Ricercatore a tempo indeterminati del settore Scientifico-disciplinare BIO10 (Biochimica). Dall'anno accademico 2002-2003 è impegnato nella didattica universitaria con corsi di Biochimica e Metodologie Biochimiche presso la Facoltà di Scienze Matematiche Fisiche e Naturali di Sapienza Università (corsi di laurea in Scienze Biologiche e Biotecnologie Agro-industriali). I suoi interessi nel campo della ricerca scientifica riguardano principalmente lo studio degli enzimi coinvolti nella biosintesi e nella regolazione della vitamina B6 e degli enzimi che utilizzano questa molecola come cofattore. Recentemente si è occupato dell'ingegnerizzazione di ceppi batterici per la produzione e l'inserimento di aminoacidi non canonici in proteine ricombinanti. È autore di più di 50 pubblicazioni su riviste scientifiche internazionali con referee e ha partecipato come curatore, autore e traduttore a vari testi di biochimica.
Dr. Martino Luigi di Salvo received his PhD in Biochemistry from the Department of Biochemistry of the University of Rome La Sapienza in 1998. He is been working as a Post-Doctoral Fellow at the Department of Biochemistry and Molecular Biophysics of Virginia Commonwealth University with Dr. Verne Schirch on the biochemistry of PLP-dependent enzymes and on the mechanism and regulation of enzymes involved in the biosynthesis of vitamin B6. From 2001 he is back at University of Rome La Sapienza with a tenured Researcher/Assistant Professor position. He currently teaches several courses of Biochemistry and Biochemical Methodologies at the School of Sciences of the same University. His research interests are mainly focused on understanding at a molecular level the structure/function relationship and the mechanism of action and regulation of a series of enzymes involved in the salvage pathways of pyridoxal 5’-phosphate in bacterial and mammalian cells. He's recently gotten involved in engineering bacterial strains for production and incorporation of noncanonical amino acids into recombinant proteins. He’s the author of more than 50 publications on international peer-reviewed journals. He’s been a author, editor and translator of several Biochemistry text books. |