• Istituto di Biologia e Patologia Molecolari

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Istitute of Molecular Biology and Pathology - National Research Council
Department of Biochemical Sciences - A. Rossi Fanelli
Sapienza University of Rome - Edificio di Chimica Biologica (CU27) - Ground Floor, lab T12, office T16 - P.le A. Moro 5, 00185 Roma

My scientific activity is focused on the structural and functional characterization of proteins involved in biological processes crucial for the development of cancer and those involved in the biosynthesis of antibiotics endowed with potential biotechnological applications. I also study how to engineer proteins to produce smart nano-vehicles for selective drug delivery for nanomedicine application. The goal of my research is identifying potential drug targets, drug makers and drug delivery proteins and defining their biomolecular profile to understand how they work and how to control their functions, using a transversal and multidisciplinary approach encompassing molecular biology, biochemistry, biophysics, structural biology and bioinformatics.

Milazzo L, Exertier C, Becucci M, Freda I, Montemiglio LC, Savino C, Vallone B, Smulevich G (2020) Lack of orientation selectivity of the heme insertion in murine neuroglobin revealed by resonance Raman spectroscopy. FEBS J; doi: 10.1111/febs.15241. [Epub ahead of print]

Ferrante C, Batignani G, Pontecorvo E, Montemiglio LC, Vos MH, Scopigno T (2020) Ultrafast dynamics and vibrational relaxation in six-coordinate heme proteins revealed by femtosecond stimulated Raman spectroscopy. J Am Chem Soc, 142(5), 2285-2292: doi: 10.1021/jacs.9b10560.

Ardiccioni C, Arcovito A, Della Longa S, van der Linden P, Bourgeois D, Weik M, Montemiglio LC, Savino C, Avella G, Exertier C, Carpentier P, Prangé T, Brunori M, Colloc’h N, Vallone B (2019) Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments. IUCrJ, 6(5), 1-11; doi: 10.1107/S2052252519008157.

Testi C, Boffi A, Montemiglio LC (2019) Structural analysis of the transferrin receptor multifaceted ligand(s) interface. Biophys Chem, 254, 106242; doi: 10.1016/j.bpc.2019.106242. 

Exertier C, Milazzo L, Freda I, Montemiglio LC, Scaglione A, Cerutti G, Parisi G, Anselmi M, Smulevich G, Savino C, Vallone B (2019) Proximal and distal control for ligand binding in neuroglobin: role of the CD loop and evidence for His64 gating. Sci Rep, 9(1), 5326; doi: 10.1038/s41598-019-41780-3.

*Montemiglio LC, *Testi C, Ceci P, Falvo E, Pitea M, Savino C, Arcovito A, Peruzzi G, Baiocco P, Mancia F, Boffi A, des Georges A, Vallone B (2019) Cryo-EM structure of the human ferritin–transferrin receptor 1 complex. Nat Commun, 10(1), 1121; doi: 10.1038/s41467-019-09098-w.

*Parisi G, *Montemiglio LC, Scaglione A, Sciara G, Savino C, Vallone B (2019) Substrate-induced conformational changes in the cytochrome P450 OleP. FASEBJ, 33(2):1787-1800; doi: 10.1096/fj.201800450RR.

Calisti L, Trabuco MC, Boffi A, Testi C, Montemiglio LC, des Georges A, Benni I, Ilari A, Taciak B, Białasek M, Rygiel T, Król M, Baiocco P, Bonamore A (2018) Engineered ferritin for lanthanide binding. PLoS One, 13(8), e0201859; doi: 10.1371/journal.pone.0201859.

Ferrante C, Batignani G, Fumero G, Pontecorvo E, Virga A, Montemiglio LC, Cerullo G, Vos MH, Scopigno T (2018) Resonant broadband stimulated Raman scattering in myoglobin. J Raman Spectrosc, 49(6), 913-920; doi: org/10.1002/jrs.5323. 

Scaglione A, Monteonofrio L, Parisi G, Cecchetti C, Siepi F, Rinaldo C, Giorgi A, Verzili D, Zamparelli C, Savino C, Soddu S, Vallone B, Montemiglio LC (2018) Effects of Y361-auto-phosphorylation on structural plasticity of the HIPK2 kinase domain. Protein Sci, 27(3), 725-737; doi: 10.1002/pro.3367.

Colloc'h N, Carpentier P, Montemiglio LC, Vallone B, Prangé T (2017) Mapping Hydrophobic Tunnels and Cavities in Neuroglobin with Noble Gas under Pressure. Biophys J, 113(10), 2199-2206; doi: 10.1016/j.bpj.2017.10.014.

Scaglione A, Fullone MR, Montemiglio LC, Parisi G, Zamparelli C, Vallone B, Savino C, Grgurina I (2017) Structure of the adenylation domain Thr1 involved in the biosynthesis of 4-chlorothreonine in Streptomyces sp. OH-5093: protein flexibility and molecular bases of substrate specificity. FEBS J, 284(18), 2981-2999; doi: 10.1016/j.biopen.2017.03.003.

*Scaglione A, *Montemiglio LC, *Parisi G, Asteriti IA, Bruni R, Cerutti G, Testi C, Savino C, Mancia F, Lavia P, Vallone B (2017) Subcellular localization of the five members of the human steroid 5α-reductase family. Biochimie Open, 4, 99-106; doi: 10.1111/febs.14163.

de Turris V, Cardoso Trabuco M, Peruzzi G, Boffi A, Testi C, Vallone B, Montemiglio LC, Georges AD, Calisti L, Benni I, Bonamore A, Baiocco P. (2017) Humanized archaeal ferritin as a tool for cell targeted delivery. Nanoscale, 9(2), 647-655; doi: 10.1039/c6nr07129e.

*Montemiglio LC, *Parisi G, Scaglione A, Sciara G, Savino C, Vallone B (2016) Functional analysis and crystallographic structure of clotrimazole bound OleP, a cytochrome P450 epoxidase from Streptomyces antibioticus involved in oleandomycin biosynthesis. Biochim Biophys Acta, 1860(3),465-475; doi: 10.1016/j.bbagen.2015.10.009.

Barteri M, De Carolis R, Marinelli F, Tomassetti G, Montemiglio LC (2016) Effects of microwaves (900 MHz) on peroxidase systems: a comparison between lactoperoxidase and horseradish peroxidase. Electromagn Biol Med, 35(2), 126-33; doi: 10.3109/15368378.2014.1002135.

- Lucia D'Auria, undergraduate student
- Andrea Mangullo, postgraduate student
- Elena Gugole, PhD student
- Ida Freda, PhD student
- Dr Cécile Exertier, Post Doc
- Dr Carmelinda Savino, CNR-IBPM Researcher
- Prof Beatrice Vallone, University of Rome Sapienza