• Istituto di Biologia e Patologia Molecolari


Tel :+39064457527
Fax :+39064457529
mario.fiore@uniroma1.it
MARIO FIORE
Research Technician

Istitute of Molecular Biology and Pathology - National Research Council
Department of Biology and Biotecnology - Charles Darwin
Sapienza University of Rome - sede di Via degli Apuli 4 00185 Roma I\u00b0floor

My past research activity as CTER has focussed on studies of the induction of cytogenetic damage by environmental agents using cytogenetic assays. I have also developed flow cytometric approaches to investigate apoptosis and cell cycle alterations induced by various types of oxidative damage (genotoxic, mitotic, oxydative)  and to evaluate the efficacy of various modulators. 

Currently my main research objective is to characterise anti-mitotic and anti-proliferative molecules which will then be selected for further preclinical and clinical assays as novel potential anti-cancer drugs: 

 - of natural origin (plant extracts from the Cuban flora), and

-  synthetic molecules designed to inhibit selected mitotic proteins, such as Hec1  (Highly expressed in cancer protein 1). 

An additional research goal is to identify the induction of alterations in the process of mitosis after exposure to environmental chemicals (e.g. pesticides). These objectives are being carried out by analysing the expression of both proliferation markers and mitotic regulators by immunofluorescence and flow cytometry.

 

I am in charge of the cell culture structure of the IBPM Laboratories at Via degli Apuli (San Lorenzo Section, Sapienza University) and of the IBPM flow cytometry facility (link). 

1. Ferretti C., P. Totta, M. Fiore, M. Mattiuzzo, T. Schillaci, R. Ricordy, A. Di Leonardo,

and F. Degrassi. (2010). Expression of the kinetochore protein Hec1 during the cell cycle in normal and cancer cells and its regulation by the pRb pathway. Cell Cycle 9:20, 4174-82.

2. Filippi S., F. Palitti, W. Martínez-López, M. Fiore and A.T. Natarajan. (2010). Relationship between DNA Repair and formation of Sister Chromatid Exchanges and  Chromatid Aberrations under the influence of Poly(ADP-Ribose) Polymerase inhibition by 3-Aminobenzamide. Cytogenet Genome Res. 128(1-3):118-23. 

R. Meschini, A. Berni, E. Marotta, S. Filippi, M. Fiore, P. Mancinelli, A.T. Natarajan

and F. Palitti. (2010) DNA Repair mechanisms involved in the removal of DBPDE-           induced lesions leading to Chromosomal alterations in CHO Cells. Cytogenet Genome Res. 128(1-3):124-30. 

4. Leone S., M. Fiore, M. G. Lauro, S. Pino, T. Cornetta and R. Cozzi. (2008) Resveratrol and X-Rays affect gap junction intercellular communications in human glioblastoma cells. Mol Carcinog. 47, 587–598

5. Mattiuzzo M., M. Fiore, R. Ricordy and F. Degrassi. (2006) Aneuploidy-inducing capacity of two widely used pesticides. Carcinogenesis 27, 2511-2518.

6. M. Fiore, F. Festa, T. Cornetta, R. Ricordy and R. Cozzi. (2005). Resveratrol affects X-ray induced apoptosis and cell cycle delay in human cells in vitro. Int J Mol Med. 15, 1005-12.

7. Mally A., G. Pepe, S. Ravoori, M. Fiore, R. Gupta, W. Dekant and P. Mosesso. (2005) DNA damage and cytogenetic effects of Ochratoxin A in rats in vivo. Chem, Res. Toxicol. 18(8):1253-61.

8. Degrassi F., M. Fiore, and F. Palitti. (2004). Chromosomal aberrations and genomic instability induced by topoisomerase-targeted antitumour drugs. Curr Med Chem Anticancer Agents.  4(4), 317-25.

9. Sanchez-Lamar A., J. L. Fuentes, G. Fonseca, N. Capiro, M. Ferrer, A. Alonzo, L. Baluja, R. Cozzi, R. De Salvia, M. Fiore and M. Llagostera. (2002). Assessment of the potential genotoxic risk of Phyllanthus orbicularis HBK aqueous extracts using in vitro and in vivo assays. Toxicol. Lett  136, 87-96

10. Fiore M., R. Zanier and F. Degrassi. (2002). Reversible G1 arrest by Dimethylsulfoxide as a new method to synchronise Chinese hamster cells. Mutagenesis. 17(5), 101-106.

Personal data

Born in 1954, Italian nationality, one daughter

 

Education  

1994: Degree in Biology, Sapienza University, Rome 

 

Positions

1991-to date: CTER (Research organization Technical  collaborator) 

1978-1991: ATP (Professional technical assistant ) 

 

Visiting stages and international collaborations

Laboratory of Mutagenesis, Sylvius Laboratories, University of Leiden (The Netherlands); 

Laboratory of Genetics, University of Uppsala ( Sweden);  

Microgravity User Supporter Center, European Space Agency,  Koln, (Germany); 

Laboratory of Toxicology, Julius-Maximilian Institut, University of Wurzburg (Germany);    

Laboratory of Genetic Toxicology, University of La  Havana (Cuba);

Laboratory of Molecular Toxicology, University of Kostanz  (Germany), supported by a three-month fellowship from CNR (May-July 1996).  

 

Teaching activities

For several years I have given monographic seminars on studies of the cell cycle and  apoptosis, Faculty of Biological Sciences, University "ROMA TRE", Rome. 

In the academic years  1998-99 and 1999-2000 I have taught an integrated Mutagenesis laboratory module in the Diploma course for Technical Environmental Operator, University "ROMA TRE", Rome.