• Istituto di Biologia e Patologia Molecolari


Tel :+390649903231
Fax :+390649902082
gianna.fiorucci@cnr.it
GIANNA FIORUCCI
Researcher

Institute of Molecular Biology and Pathology - National Research Council
Istituto Superiore di Sanita - Dept. of Infectious Diseases
Istituto Superiore di Sanita - Dept. of Infectious Diseases, Building 1, floor E, room 9 - Viale Regina Elena, 299-00161 Rome, Italy

Research project focuses on the antiproliferative effect of type I Interferons (IFNs) in human keratinocytes transformed by mucosal and cutaneous Human Papilloma Viruses (HPV) E6 and E7 proteins, which target and inhibit p53 and pRb tumor suppressive proteins. The study aims to analyse the involvement of virus and cell-dependent target pathways, to identify the molecular mechanisms of type I IFNs antitumor action as well as the oncogenic potential of various HPV genotypes. HPVs are small DNA viruses involved in the development of both benign and malignant lesions localised in different anatomic districts and their life cicle appears strictly correlated to the differentiation program of keratinocytes. To date, the causative association of mucosal high risk-HPVs and cervical carcinoma has been clearly demonstrated. On the contrary, a direct role of the cutaneous beta HPV types in cancer development remains to be proven.
More recently, the research project is focusing on the analysis of miRNAs expression profile and on the characterization of the target genes regulated by the identified miRNAs in order to shed light on the molecular mechanisms underlying the oncogenic transformation of keratinocytes by mucosal and cutaneous HPVs. 
Since there is emerging evidence that cancer cell-derived exosomes may contribute to the recruitment and reprogramming of the tumor microenvironment to form a protumorigenic soil, our research has been extended to the study of these microvesicles. The possibility that HPV-infected cells, by delivering E6 and E7 oncogenes as well as HPV-deregulated miRNAs to the extracellular matrix, can transfer their oncogenic potential to the surrounding not infected cells has been investigated in our studies highlighting the importance of miRNAs and exosomes in diagnostic studies and their role as useful biomarkers.
 

Chiantore M.V., Mangino G., Iuliano M., Zangrillo M.S., De Lillis I., Vaccari G., Accardi R., Tommasino M., Fiorucci G. *, Romeo G.
IFN-beta antiproliferative effect and miRNA regulation in Human Papilloma Virus E6- and E7 transformed keratinocytes.
Cytokine 89: 235-238, doi 10.1016/j.cyto.2015.12.014. [Epub ahead of print], 2017.
(*Fiorucci and Romeo contributed equally to this study).

 

Chiantore M.V., Mangino G., Iuliano M., Zangrillo M.S., De Lillis I., Vaccari G., Accardi R., Tommasino M., Cabezas S.C., Federico M., Fiorucci G. *, Romeo G.
Human papillomavirus E6 and E7 oncoproteins affect the expression of cancer-related microRNAs: additional evidence in HPV-induced tumorigenesis.
J. Cancer Res. Clin. Oncol. 142 (8): 1751-1763, June 14: doi 10.1007/s00432-016-2189-1
[Epub ahead of print], 2016.
(*Fiorucci and Romeo contributed equally to this study).

Mangino G., Chiantore M.V., Iuliano M., Fiorucci G., Romeo G.
Inflammatory microenvironment and human papillomavirus-induced carcinogenesis.
Cytokine Growth Factor Rev. 30: 103-111, Mar 18: doi 10.1016/j.cytogfr.2016.03.007
[Epub ahead of print], 2016.


Iuliano M., Mangino G., Chiantore M.V., Fiorucci G., Accardi R., Tommasino M., Romeo G.
The role of microenvironment in tumorigenesis: Focus on dendritic cells in human papillomavirus E6, E7-transformed keratinocytes.
Cancer Cell & Microenvironment 2: e874, Epub. July 06, 2015.


Mangino G., Famiglietti M., Capone C., Veroni C., Percario Z.A. , Leone S., Fiorucci G., Lülf  S., Romeo G., Agresti C., Persichini T. and Affabris E.
HIV-1 Myristoylated Nef Treatment of Murine Microglial Cells Activates Inducible Nitric Oxide Synthase, NO2 Production and Neurotoxic Activity.
PloS One 10 (6): e0130189, Epub. June 11, 2015.


Chiantore M.V., Mangino G., Zangrillo M.S., Iuliano M., Affabris E., Fiorucci G. and G. Romeo.
Role of the Microenvironment in Tumourigenesis: Focus on Virus-Induced Tumors.
Curr. Med. Chem. 22 (8): 958-974, 2015.

Fiorucci G.
, Chiantore M.V., Mangino G., Romeo G.
MicroRNAs in virus-induced tumorigenesis and IFN system.
Cytokine Growth Factor Rev. 26 (2): 183-194, [Epub ahead of print: 2014 Nov 13], 2015.


Chiantore M.V., Vannucchi S., Accardi R., Tommasino M., Percario Z.A., Vaccari G., Affabris E., Fiorucci G. and Romeo G.
Interferon-beta Induces Cellular Senescence in Cutaneous Human Papilloma Virus-Transformed Human Keratinocytes by Affecting p53 Transactivating Activity.
PloS One, 7 (5): e36909, May 2012.


Fiorucci G.
, Chiantore M.V., Mangino G., Percario Z.A., Affabris E. and  Romeo G.
Cancer Regulator MicroRNA: Potential Relevance in Diagnosis, Prognosis and Treatment of Cancer.
Curr. Med. Chem. 19 (4): 461-474, 2012.


Percario Z.A., Mangino G., Gallo V., Chiantore M.V., Fiorucci G., Romeo G. and Affabris E.
HIV-1 Nef Transfer and Intracellular Signalling in Uninfected Cells. In “ HIV-Host Interactions”, Ed. Theresa L. Chang; Published by InTech, Janeza Trdine 9, 51000 Rijeka, 
Croatia: 61-78, 2011.


Mangino G., Percario Z.A., Fiorucci G., Vaccari G., Acconcia F., Chiarabelli C., Leone S., Noto A., Horenkamp F.A., Manrique S., Romeo G., Polticelli F., Geyer M. and Affabris E.
HIV-1 Nef induces proinflammatory state in macrophages through its acidic cluster domain: involvement of TNF alpha Receptor Associated Factor 2.
PLoS One 6 (8): e22982. doi: 10.1371/journal.pone.0022982, 2011.


Chiantore M.V., Vannucchi S., Mangino G., Percario Z.A., Affabris E., Fiorucci G. and Romeo G.  
Senescence and Cell Death Pathways and Their Role in Cancer Therapeutic Outcome.
Curr. Med. Chem. 16: 287-300, 2009.


Fiorucci G.
, Olivetta E, Chiantore M.V., and Federico M.
Microarray analysis reveals CCL24/eotaxin-2 as an effector of the pathogenetic effects induced by HIV-1 nef.
Curr. Drug Discov. Technol. 4 (1): 12-23, 2007. 


Mangino G., Percario Z. A., Fiorucci G., Vaccari G., Manrique S., Romeo G., Federico M., Geyer M., and Affabris E.
In vitro treatment of human monocyte/macrophages with myristoylated recombinant Nef leads to the activation  of MAPKs, IkB kinases and Interferon Regulatory Factor 3 and to the release of Beta Interferon.
J.Virol. 81 (6): 2777-2791, 2007.


Vannucchi S., Chiantore M.V., Mangino G., Percario Z.A., Affabris E., Fiorucci G., Romeo G.
Perspectives in Biomolecular Therapeutic Intervention in Cancer: from the Early to the New Strategies with Type I Interferons.
Curr. Med. Chem. 14 (6): 667-679, 2007.


Vannucchi S., Chiantore M.V., Fiorucci G., Percario Z.A., Leone S., Affabris E., Romeo G.
TRAIL is a key target in S-phase slowing-dependent apoptosis induced by interferon-beta in cervical carcinoma cells.
Oncogene 24: 2536-2546, 2005.


Fiorucci G.
, Vannucchi S., Chiantore Maria V., Percario Zulema A., Affabris E., Romeo G.
TNF-Related Apoptosis-Inducing Ligand (TRAIL) as a Pro-Apoptotic Signal Transducer with Cancer Therapeutic Potential.
Curr. Pharm. Design 11: 933-944, 2005.


Percario Z.A., Olivetta E., Fiorucci G., Mangino G., Peretti S. Romeo G., Affabris E., Federico M.
HIV-1 Nef Activates STAT3 in Primary Human Monocyte/Macrophages through the Release of Soluble Factors: Involvement of Nef Domains Interacting with the Cell Endocytotic Machinery.
J. Leukoc.Biol. 74 (5): 821-32, 2003.


Olivetta E., Percario Z.A., Fiorucci G., Mattia G., Schiavoni I., Dennis C., Jäger J., Harris M., Romeo G., Affabris E., Federico M.
HIV-1 Nef Induces the Release of Inflammatory Factors from Human Monocyte/ Macrophages: Involvement of Nef Endocytotic Signals and NF-kB Activation.
J. Immunol.170: 1716-1727, 2003.


Romeo G., Fiorucci G., Chiantore M.V., Percario Z.A., Vannucchi S., Affabris E.
IRF-1 as a negative regulator of cell proliferation.
J. Interferon Cytokine Res. 22: 39-47, 2002.


Federico M., Percario Z.A., Olivetta E., Fiorucci G., Muratori C., Micheli A., Romeo G.,  Affabris E.
Human immunodeficiency virus type I Nef activates STAT1 in human monocytes/macrophages through the release of soluble factor(s).
Blood 98 (9): 2752-2761, 2001.


Vannucchi S., Percario Z.A., Chiantore M.V., Matarrese P., Chelbi-Alix M.K., Fagioli M.,  Pelicci, P.G., Malorni W., Fiorucci G., Romeo G., Affabris E.
Interferon beta induces S-phase slowing via up-regulated expression of PML in squamous carcinoma cells. 
Oncogene 19: 5041-5043, 2000.

Research group:

Prof. Giovanna Romeo
Dr. Maria Vincenza Chiantore
Dr. Giorgio Mangino
Dr. Marco Iuliano

 

Research area

 HPVs, oncoproteins, IFN, microRNAs, exosomes

Collaborations with other Institutions 

- Prof. Giovanna Romeo, Sapienza University of Rome, Dept. of Medical Surgical Sciences and Biotechnologies 

- Prof. Elisabetta Affabris, Università di Roma Tre, Dept. of Biology

- Prof. Massimo Tommasino, Infections and Cancer Biology Group, International Agency for Research on Cancer-WHO (IARC), Lyon, France

- Dr. Maria Vincenza Chiantore, Istituto Superiore di Sanità, Dept. of Infectious Diseases

- Dr Giorgio Mangino, Sapienza University of Rome, Dept. of Medical Surgical Sciences and Biotechnologies
- Dr. Marco Iuliano, Sapienza University of Rome, Dept. of Medical Surgical Sciences and Biotechnologies

- Dr. Zulema Antonia Percario, Università di Roma Tre, Dept. of Biology 

- Dr. Luisa Accardi, Istituto Superiore di Sanità, Dept. of Infectious Diseases

- Dr. Paola Di Bonito, Istituto Superiore di Sanità, Dept. of Infectious Diseases

- Dr. Gabriele Vaccari, Istituto Superiore di Sanità, Dept. of Food Safety and Veterinary Public Health 

- Dr Maurizio Federico, Istituto Superiore di Sanità, National Centre for Global Health
- Dr. Fabiola Luzi, IFO - S. Gallicano

First name                                          Gianna

Last name                                           Fiorucci

Nationality                                          Italian

Place and date of birth                          Rome  -  June 24th, 1955

E-mail                                                gianna.fiorucci@cnr.it

 

 

EDUCATION AND TRAINING

 

From 1978 to 1980  Ph.D student at the Institute of Zoology, Sapienza University of Rome, Faculty of Mathematics, Physics and Natural Sciences 

 

March 26, 1980  Ph.D in Biological Sciences

 

from June 1980 to August 1981  Post-doctoral position at the Institute of Human Genetics, Università  Cattolica del Sacro Cuore of Rome

 

from September 1981 to August 1990  Post-doctoral fellow, Consiglio Nazionale delle Ricerche – Rome, Italy

 

 

PROFESSIONAL EXPERIENCE

 

From 31 December 2006 to date  Researcher at the Institute of Molecular Biology and Pathology, Consiglio Nazionale delle Ricerche – Rome, Italy

 

from February 2003 to 30 December 2006   Technologist at the Institute of Molecular Biology and Pathology, Consiglio Nazionale delle Ricerche – Rome, Italy

 

from September 1990 to February 2003  Technologist at the Institute of Biomedical Technologies, Consiglio Nazionale delle Ricerche – Rome, Italy

 

from March 2003 to December 2016  Guest scientist at the Dept. of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità–Rome, Italy

from January 2017 to date  Guest scientist at the Dept. of Infectious Diseases, Istituto Superiore di Sanità–Rome, Italy 


from April 1983 to February 2003   Guest scientist at Laboratory of Virology, Istituto Superiore di Sanità – Rome, Italy

 

from July 1986 to June 1987   Research fellow at the Institute of Cancer Research (London, Great Britain) Dept. of Cell and Molecular Biology, in Dr. Alan Hall’s Laboratory

 

from January 1985 to June 1986  Fellow of Italian Association of Cancer Research (AIRC) at the Institute of Cancer Research (London, Great Britain) Dept. of Cell and Molecular Biology, in Dr. Alan Hall’s laboratory

 

from January 1983 to March 1983  Guest scientist at the National Cancer Institute (Bethesda, MD, U.S.A.) Laboratory of Pathophysiology, in Dr. Barbara Vonderhaar’s laboratory



RESEARCH PROJECTS

Co-applicant of the CNR-Agenzia 2000 Grant entitled “Regolazione trascrizionale del ciclo cellulare: ruolo di Rb e PML”

Co-applicant of the Progetto di ricerca finalizzata 2002-03 Ministero della Salute Grant entitled ”Strategie di immunoterapia contro genotipi di HPV oncogeni e non oncogeni.”

Co-applicant of the MIUR, PRIN 2005 Grant entitled "Development of new therapeutic strategies against human herpesvirus and papilloma virus infections and their diseases."

Co-applicant of the MIUR, PRIN 2008 Grant entitled "Studies on the molecular mechanisms of Human Papilloma Viruses infection and associated diseases to develop novel therapeutic strategies.

Co-applicant of the Research Project Sapienza "Ateneo Federato" Grant "Ricerche Universitarie", "Grandi Apparecchiature" a.a. 2005-2011.

Co-applicant  in various AIRC, AIDS and MIUR projects 

 

Membership: International Society for Interferon and Cytokine Research since 1989